Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE

文献类型： 外文期刊

第一作者： Zhang, Yanping

作者： Zhang, Yanping;Yu, Huaping;Han, Jian;Song, Changnian;Fang, Jinggui;Yu, Mingliang;Ma, Ruijuan

作者机构：

关键词： Prunus persica;microRNAs;Computational prediction;qRT-PCR;miR-RACE

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Twenty-two potential miRNAs from seven miRNA families were first predicted from more than 80,857 EST sequences of peach (Prunus persica). Using two specific 50 and 30 miRNA RACE (miR-RACE) PCR reactions and sequence-directed cloning, we accurately determined the precise sequences, especially both ends, of eight candidate miRNAs. The sequencing results demonstrated that the ppe-miRNAs were conserved to those that were predicted computationally except ppe-miR171b. We validated the existence of two members (ppe-miR171a and miR171b) of the miR171 family in peach that belonged to different precursors. qRT-PCR was further employed in analyzing expression of the eight miRNAs in peach leaves, flowers, and fruits at different developing stages, where some of the miRNAs showed tissue-specific expression.

分类号： Q7

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