Molecular phylogenetic diversity of Bacillus community and its temporal-spatial distribution during the swine manure of composting

文献类型: 外文期刊

第一作者: Wu, Jian

作者: Wu, Jian;Yi, Jing;Deng, Chang-Yan;Zheng, Rong;Chao, Zhe;Yi, Jing;Deng, Chang-Yan;Zheng, Rong;Chao, Zhe;Wu, Hua-Yu

作者机构:

关键词: Bacillus;Composting;DGGE;Diversity;RFLP;Temporal-spatial distribution

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In order to obtain the diversity and temporal-spatial distribution of Bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and -denaturing gradient gel electrophoresis (PCR-DGGE). Bacillus species were firstly isolated from the composting. Based on temperature changes, the temporal-spatial characteristics of total culturable Bacillus were remarkable that the number of the culturable Bacillus detected at the high-temperature stage was the highest in each layer of the pile and that detected in the middle layer was the lowest at each stage of composting respectively. The diversity of cultivated Bacillus species isolated from different composting stages was low. A total of 540 isolates were classified by the RFLP method and partial 16S rDNA sequences. They affiliated to eight species including Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus megaterium, Bacillus licheniformis, Bacillus pumilus, and Bacillus circulans. The predominant species was B. subtilis, and the diversity of culturable Bacillus isolated in the middle-level samples at temperature rising and cooling stages was the highest. The DGGE profile and clone library analysis revealed that the temporal-spatial distribution of Bacillus community was not obvious, species belonging to the Bacillus were dominant (67%) with unculturable bacteria and B. cereus was the second major culturable Bacillus species. This study indicated that a combination of culture and culture-independent approaches could be very useful for monitoring the diversity and temporal-spatial distribution of Bacillus community during the composting process.

分类号: Q939.9

  • 相关文献

[1]Decline in extractable kitasamycin during the composting of kitasamycin manufacturing waste with dairy manure and sawdust. Ding, Nengfei,Liu, Chen,Fu, Qinglin,Guo, Bin,Li, Hua,Li, Ningyu,Lin, Yicheng,Li, Weidong. 2014

[2]Response of compost maturity and microbial community composition to pentachlorophenol (PCP)-contaminated soil during composting. Zeng, Guangming,Yu, Zhen,Chen, Yaoning,Zhang, Jiachao,Li, Hui,Zhao, Mingjie,Zeng, Guangming,Yu, Zhen,Chen, Yaoning,Zhang, Jiachao,Li, Hui,Zhao, Mingjie,Yu, Man.

[3]A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Peng, DL,Moens, M.

[4]Analysis of Clostridium cluster I community diversity in pit mud used in manufacture of Chinese Luzhou-flavor liquor. Tang, Yuming,Ren, Daoqun,Yao, Wanchun,Tian, Xinhui,Zhang, Xinyu,Zhao, Ke,Yi, Bin,Deng, Bo. 2015

[5]Analysis of Fungal Diversity in the Composting by Sequencing of Cloned PCR-Amplified 18S rDNA and Denaturing Gradient Gel Electrophoresis. Li, Wan,Xiao, Jialei,Lai, Yongcai,Xu, Xiuhong,Li, Hongtao,Zhang, Bixian. 2012

[6]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[7]The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria. Chen, Y.,Zhu, W.,Li, J.,Mo, Z.,Xiao, P.. 2016

[8]Isolation and characterisation of H2O2-decomposing bacteria from Antarctic seawater. Wang, Wei,Liu, Wan-Shun,Wang, Wei,Sun, Mi. 2008

[9]Effects of dietary medicinal herbs and Bacillus on survival, growth, body composition, and digestive enzyme activity of the white shrimp Litopenaeus vannamei. Yu, Ming-Chao,Li, Zhuo-Jia,Lin, Hei-Zhao,Wen, Guo-Liang,Ma, Shen.

[10]Enhanced production of a thermostable mannanase by immobilized cells of Bacillus subtilis on various membranes. Wu, Aimin,Jiang, Zhengqiang,Tang, Luo,Yan, Qiaojuan,Bo, Shi.

[11]Analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial Bacillus amyloliquefaciens SQR9. Shao, Jiahui,Li, Shuqing,Zhang, Guishan,Zhang, Ruifu,Shao, Jiahui,Li, Shuqing,Zhang, Nan,Cui, Xiaoshuang,Zhou, Xuan,Shen, Qirong,Zhang, Ruifu,Shao, Jiahui,Li, Shuqing,Zhang, Nan,Cui, Xiaoshuang,Zhou, Xuan,Shen, Qirong,Zhang, Ruifu. 2015

[12]Bacillus amyloliquefaciens GB1 can effectively control apple valsa canker. Zhang, Jun-xiang,Gu, Yan-bing,Chi, Fu-mei,Ji, Zhi-rui,Wu, Jian-yuan,Dong, Qing-long,Zhou, Zong-shan.

[13]Isolation and characterization of chromium(VI)-reducing Bacillus sp FY1 and Arthrobacter sp WZ2 and their bioremediation potential. Xiao, Wendan,Ye, Xuezhu,Sun, Caixia,Zhang, Qi,Xu, Ping,Yang, Xiaoe,Zhu, Zhiqiang.

[14]PhoR sequences as a phylogenetic marker to differentiate the species in the Bacillus subtilis group. Guo, Qinggang,Li, Shezeng,Lu, Xiuyun,Li, Baoqing,Dong, Weixin,Ma, Ping,Stummer, Belinda.

[15]Contribution of Bacillus Isolates to the Flavor Profiles of Vanilla Beans Assessed through Aroma Analysis and Chemometrics. Gu, Fenglin,Chen, Yonggan,Fang, Yiming,Wu, Guiping,Tan, Lehe,Chen, Yonggan,Chen, Yonggan.

[16]Cloning, overexpression, and functional characterization of a phytase from the genus Bacillus. Li, Zhengang,Zhao, Aichun,Wang, Xiling,Jin, Xiaoyun,Li, Jun,Yu, Maode,Li, Zhengang.

[17]Genetic differentiation of wild relatives of rice as assessed by RFLP analysis. Lu, BR,Zheng, KL,Qian, HR,Zhuang, JY. 2002

[18]QTL mapping of resistance to Sporisorium reiliana in maize. Lubberstedt, T,Xia, XC,Tan, G,Liu, X,Melchinger, AE. 1999

[19]Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Larkin, PJ. 2001

[20]Identification of molecular markers for the Thinopyrum intermedium chromosome 2Ai-2 with resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Lin, ZS,Chen, X,Wang, XP. 2000

作者其他论文 更多>>