Molecular phylogenetic diversity of Bacillus community and its temporal-spatial distribution during the swine manure of composting
文献类型: 外文期刊
第一作者: Wu, Jian
作者: Wu, Jian;Yi, Jing;Deng, Chang-Yan;Zheng, Rong;Chao, Zhe;Yi, Jing;Deng, Chang-Yan;Zheng, Rong;Chao, Zhe;Wu, Hua-Yu
作者机构:
关键词: Bacillus;Composting;DGGE;Diversity;RFLP;Temporal-spatial distribution
期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In order to obtain the diversity and temporal-spatial distribution of Bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and -denaturing gradient gel electrophoresis (PCR-DGGE). Bacillus species were firstly isolated from the composting. Based on temperature changes, the temporal-spatial characteristics of total culturable Bacillus were remarkable that the number of the culturable Bacillus detected at the high-temperature stage was the highest in each layer of the pile and that detected in the middle layer was the lowest at each stage of composting respectively. The diversity of cultivated Bacillus species isolated from different composting stages was low. A total of 540 isolates were classified by the RFLP method and partial 16S rDNA sequences. They affiliated to eight species including Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus megaterium, Bacillus licheniformis, Bacillus pumilus, and Bacillus circulans. The predominant species was B. subtilis, and the diversity of culturable Bacillus isolated in the middle-level samples at temperature rising and cooling stages was the highest. The DGGE profile and clone library analysis revealed that the temporal-spatial distribution of Bacillus community was not obvious, species belonging to the Bacillus were dominant (67%) with unculturable bacteria and B. cereus was the second major culturable Bacillus species. This study indicated that a combination of culture and culture-independent approaches could be very useful for monitoring the diversity and temporal-spatial distribution of Bacillus community during the composting process.
分类号: Q939.9
- 相关文献
作者其他论文 更多>>
-
Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds
作者:Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian
关键词:Grassland red cattle; yanbian cattle; yan Yellow cattle; intrinsic factor-cobalamin receptor; MIA SH3 domain ER-derived factor 3; copy number variations
-
An Ultra-Sensitive Quarantine Pathogen On-Site Detection Based on a One-Pot Asymmetric Recombinase Polymerase Amplification and MNAzyme-Assisted Target Recycling Biosensor (OAR-MNA)
作者:Yang, Lei;Peng, Cheng;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Wang, Xiaofu;Xu, Junfeng;Chen, Guanwei;Yan, Jiatong;Sun, Meihao;Bo, Yongming;Fang, Xiaoxue;Wu, Jian
关键词:asymmetric RPA; bacterial fruit blotch; cucumber green mottle mosaic virus; MNAzyme; one-pot; ssDNA
-
Unraveling the genetic diversity and adaptive traits of laboratory pig breeds within the perspective of whole - genome resequencing
作者:Yuan, Haonan;Zhao, Shengguo;Yang, Yanan;Quan, Jinqiang;Li, Changwen;Xia, Changyou;Gao, Caixia;Chao, Zhe
关键词:Animal models; Local experimental pigs; Genetic variations; Selection signatures; Stopgain
-
Aspartate Metabolism-Driven Gut Microbiota Dynamics and RIP-Dependent Mitochondrial Function Counteract Oxidative Stress
作者:Jin, Shunshun;Wang, Chenyu;He, Yiwen;Zhou, Hui;He, Xinmiao;He, Liuqin;Wu, Jian;Wang, Chenyu;He, Yiwen;Tang, Yulong;Huang, Le;Zhou, Hui;Peng, Can;Li, Tiejun;Yin, Yulong;He, Liuqin;Jin, Shunshun;Wu, Ziping;Liu, Di;Feng, Yanzhong;Chen, Heshu;He, Xinmiao;Yang, Guan;Qiu, Jiazhang;Yin, Yulong
关键词:aspartate; microbiota; oxidative stress; RIP pathway
-
The giant genome of lily provides insights into the hybridization of cultivated lilies
作者:Liang, Yuwei;Gao, Qiang;Zhang, Liangsheng;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Du, Yunpeng;Pan, Wenqiang;Zhang, Xiuhai;Zhang, Mingfang;Wu, Jian;Pan, Wenqiang;Wang, Shaokun;Song, Xiaoming;Zhong, Linlin;Zhang, Fan;Li, Yan;Wang, Zhiwei;Li, Danqing;Gu, Yang;Chen, Zhong-Hua;Chen, Zhong-Hua;Mayer, Klaus F. X.;Mayer, Klaus F. X.;Zhou, Xiaofan;Zhang, Liangsheng;Zhang, Liangsheng
关键词:
-
Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops
作者:Ding, Lin;Wang, Xiaofu;Chen, Xiaoyun;Xu, Xiaoli;Wei, Wei;Yang, Lei;Ji, Yi;Xu, Junfeng;Peng, Cheng;Wu, Jian
关键词:Dual CRISPR; Genetically Modified (GM) detection; One-pot reaction; Recombinase polymerase amplification; (RPA); Multiplexed lateral flow strips
-
METTL14 regulates proliferation and differentiation of duck myoblasts through targeting MiR-133b
作者:Jiang, Qicheng;Zhou, Hailong;Xu, Tieshan;Xiao, Zhepeng;Xing, Lingjing;Zheng, Xinli;Chao, Zhe;He, Zhongchun;Gu, Lihong;Yu, Ping;Yang, Wei
关键词: