Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection

文献类型： 外文期刊

第一作者： Gao, Kun

作者： Gao, Kun;Qian, Heying;Wu, Ping;Qin, Guangxing;Guo, Xijie;Gao, Kun;Qian, Heying;Wu, Ping;Qin, Guangxing;Guo, Xijie;Gao, Kun;Deng, Xiangyuan

作者机构：

关键词： Bombyx mori;Cytoplasmic polyhedrosis virus;Expression analysis;Ganglioside-induced differentiation-associated protein 1;Midgut;Rapid amplification of cDNA ends

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel ganglioside-induced differentiation-associated protein 1 gene (BmGDAP1) was first cloned and sequenced from silkworm, Bombyx mori using rapid amplification of cDNA ends (RACE). The full-length cDNA of BmGDAP1 was 1514. bp, consisting of a 91. bp 5' untranslated region (UTR), a 424. bp 3'-UTR and a 999. bp open reading frame (ORF). The ORF encoded a polypeptide of 332 amino acids, which possessed a thioredoxin (TRX)-like domain, a glutathione S-transferase-C (GST-C) family domain and a transmembrane segment. Furthermore, quantitative real-time PCR analysis revealed that BmGDAP1 transcripts were mainly presented in the tissues of hemocytes and midgut of silkworm, and its expression level was down-regulated in the hemocytes, while up-regulated in the midgut. Therefore, it could be concluded that BmGDAP1 plays an important role in the recognition and immune response of silkworm to BmCPV infection.

分类号： R394

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