Simultaneous Detection of Three Viroid Species Infecting Hops in China by Multiplex RT-PCR
文献类型: 外文期刊
第一作者: Lu, Wenxia
作者: Lu, Wenxia;Xu, Pinsan;Lu, Wenxia;Zhang, Zhixiang;Jiang, Dongmei;Li, Shifang;Liu, Shengxue;Wang, Hongqing
作者机构:
关键词: Hops;Humulus lupulus;viroids;HLVd;HSVd;AFCVd;China
期刊名称:JOURNAL OF PHYTOPATHOLOGY ( 影响因子:1.789; 五年影响因子:1.574 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: We have developed a multiplex RT-PCR protocol for the simultaneous detection of three viroids in three different genera that infect hops: Hop latent viroid (HLVd; Cocadviroid), Hop stunt viroid (HSVd; Hostuviroid) and Apple fruit crinkle viroid (AFCVd; Apscaviroid). The method was validated by testing 175 hop samples collected from the Xinjiang autonomous region of China. All samples were found to be positive for HLVd but negative for AFCVd, confirming the widespread or even ubiquitous infection of HLVd and the low incidence of AFCVd in hops in China. In addition, HSVd was detected in 22.86% of the samples tested. This rapid and reliable multiplex RT-PCR assay provides an effective method for detection of three important viroid species in large-scale surveys for disease management in hops.
分类号: Q945
- 相关文献
作者其他论文 更多>>
-
Molecular and Biological Characteristics of a Peach Latent Mosaic Viroid PC Isolate in Peach from China: Base Mutations in Hairpin Stems and Implications for Symptomatology
作者:Lu, Meiguang;Zhang, Zimeng;Huang, Wen;Zhang, Zhixiang;Li, Shifang;Zhou, Jun
关键词:base mutation; hairpin insertion; PC variant; peach latent mosaic viroid; PLMVd infectivity; symptomatology; topping treatment
-
Knockout of SlDCL2b attenuates the resistance of tomato to potato spindle tuber viroid infection
作者:Zhang, Yuhong;Tian, Xiaxia;Xu, Huiyuan;Zhan, Binhui;Li, Shifang;Zhang, Zhixiang;Tian, Xiaxia;Zhou, Changyong
关键词:dicer proteins; microRNA; RNA silencing; siRNA; tomato; viroid
-
Plant virology in the 21st century in China: Recent advances and future directions
作者:Wu, Jianguo;Wei, Taiyun;Li, Yi;Zhang, Yongliang;Wang, Xianbing;Li, Dawei;Zhang, Yongliang;Wang, Xianbing;Li, Dawei;Li, Fangfang;Li, Shifang;Zhou, Xueping;Zhang, Xiaoming;Cui, Feng;Zhang, Xiaoming;Ye, Jian;Cui, Feng;Zhang, Lili;Ye, Jian;Zhang, Lili;Li, Zhenghe;Zhou, Xueping;Tao, Xiaorong;Yan, Fei;Liu, Yule;Li, Yi
关键词:China; emerging plant viruses; plant virology; plant resistance/susceptibility to viral infection; plant-virus transmission vector interactions; plant virus-based vectors; viral pathogenesis
-
Microfungi Associated with Peach Branch Diseases in China
作者:Zhou, Ying;Li, Shifang;Zhou, Ying;He, Zhizheng;Zhang, Wei;Liu, Mei;Song, Jinyan;Yan, Jiye;Zhou, Ying;Fan, Zaifeng;Manawasinghe, Ishara S.
关键词:diversity; peach diseases; morphology; phylogenetic analyses; new species; new records
-
Mechanism of improving the quality of table grapes (Vitis. spp.) by exogenous eugenol based on transcriptome
作者:Jiang, Nan;Wang, Liuqing;Gao, Yuan;Li, Qianqian;Jiang, Dongmei;Wang, Meng;Jiang, Nan;Yao, Weirong;Jiang, Nan;Yao, Weirong
关键词:Eugenol; grape; postharvest quality; transcriptome
-
Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta
作者:Du, Mingyi;Xu, Kaijie;Huang, Yudi;Xu, Yizhu;Wen, Wenlin;Zhang, Zhixiang;Xu, Hanhong;Wu, Xinzhou;Du, Mingyi;Yin, Zhibin
关键词:insecticide; spatial metabolomics; metabolic disturbance; toxicology; S; invicta
-
Seroprevalence of Chlamydia abortus and Brucella spp. and risk factors for Chlamydia abortus in pigs from China
作者:Zhang, Huaiyu;Zhang, Zhixiang;Li, Yunhui;Tong, Dewen;Li, Wei;Jin, Youshun;Li, Zhaocai;Zhou, Jizhang
关键词:Chlamydia abortus; Brucella spp.; Seroprevalence; Abortion; Pig; China