Effect of vitrification on promoter methylation and the expression of pluripotency and differentiation genes in mouse blastocysts

文献类型： 外文期刊

第一作者： Zhao, Xue-Ming

作者： Zhao, Xue-Ming;Du, Wei-Hua;Hao, Hai-Sheng;Wang, Dong;Qin, Tong;Liu, Yan;Zhu, Hua-Bin

作者机构：

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The present study was designed to determine the effects of vitrification on promoter methylation and the expression levels of pluripotency and differentiation genes in mouse blastocysts. Promoter region CpG methylation patterns and the expression levels of octamer-binding transcription factor (Oct4), Nanog homeobox (Nanog), caudal-type homeobox 2 (Cdx2), and heart and neural crest derivatives-expressed transcript 1 (Hand1) were analyzed in fresh and vitrified mouse blastocysts. Methylation was measured by bisulphate mutagenesis and sequencing; gene expression was determined by real-time reverse transcription-PCR. The results showed that vitrification significantly reduced the methylation levels of the Oct4 (85% vs. 62.5%), Nanog (77.5% vs. 55%), and Cdx2 promoters (4.6% vs. 1.4%; P??0.05). The expression levels of Cdx2 and Hand1 were not significantly different in fresh and vitrified blastocysts. In conclusion, vitrification significantly decreased Oct4, Nanog, and Cdx2 promoter methylation in mouse blastocysts, which correlated with increased expression of Oct4 and Nanog. Mol. Reprod. Dev. 79:445450, 2012

分类号： Q7

  • 相关文献
作者其他论文 更多>>

微信小程序