Polyethylene glycol-mediated transformation of fused egfp-hph gene under the control of gpd promoter in Pleurotus eryngii
文献类型: 外文期刊
第一作者: Yin, Yonggang
作者: Yin, Yonggang;Liu, Yu;Wang, Shouxian;Zhao, Shuang;Geng, Xiaoli;Xu, Feng;Yin, Yonggang;Li, Ming;Jin, Haojie
作者机构:
关键词: Pleurotus eryngii;Polyethylene glycol;Promoter;Protoplasts;Transformation efficiency
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
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收录情况: SCI
摘要: Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the control of the strong and constitutive native gpd promoter from P. eryngii. The recombinant plasmid was used to transform of P. eryngii protoplasts. Successful transformation was demonstrated by molecular analyses. Moreover, the mycelia of the transformants showed green epipolic dispersion on fluorescence microscopy. About 90-210 transformants were produced per mug plasmid DNA per 10~7 viable protoplasts.
分类号: Q7
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