Use of a culture independent method to analyze the diversity of soil fungi surrounding Chroogomphus rutilus in the Beijing region of China

文献类型： 外文期刊

第一作者： Liu, Yu

作者： Liu, Yu;Wang, Shouxian;Yin, Yonggang;Zhao, Shuang;Wang, Peng;Geng, Xiaoli;Xu, Feng;Yin, Yonggang;Li, Ming;Jin, Haojie

作者机构：

关键词： Chroogomphus rutilus;Internal transcribed spacer;Metagenomic DNA;Restriction fragment length polymorphism

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Large-scale cultivation of Chroogomphus rutilus is too inefficient to be commercially feasible. In addition, isolating C. rutilus mycelia in the wild is difficult. Thus, determining the natural habitat of its fruiting body is important. The present study focused on the ecology of the C. rutilus habitat to facilitate its large-scale cultivation. A culture-independent molecular approach-a powerful technology for microbiological ecology studies-was used to investigate the diversity of soil fungal communities in samples surrounding C. rutilus from the Beijing region of China. Metagenomic DNA was isolated from soil samples collected around C. rutilus, and an internal transcribed spacer (ITS) gene library was constructed. Subsequently, polymerase chain reaction products were digested with HinfI, HaeIII, MspI, TaqI, or MboI. Clones were selected and sequenced based on their restriction fragment length polymorphisms. The diversity of the fungi represented by their ITS sequences was analyzed. Our results indicate the presence of numerous fungi in the C. rutilus habitat. This study is the first demonstration of the fungal ecology surrounding C. rutilus using a culture independent method.

分类号： Q93

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