An Efficient Identification of 68 Apple Cultivars Using a Cultivar Identification Diagram (CID) Strategy and RAPD Markers

文献类型： 外文期刊

第一作者： Wang, Wenyan

作者： Wang, Wenyan;Fang, Jinggui;Wang, Kun;Liu, Fengzhi

作者机构：

关键词： fruit industry;longer primer;molecular marker;polymorphic band

期刊名称：KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY （ 影响因子：0.589； 五年影响因子：0.443 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The study aimed to establish an efficient tool for cultivar identification and characterization being the first steps of apple introduction and improvement program. We utilized a method to efficiently record DNA molecular fingerprints of plant individuals genotyped by RAPD, which could be used as efficient reference information for quick plant identification. Ten of sixty 11-mer primers were screened to identify the 68 apple genotypes which could be distinguished by a combination of several primers.All cultivars were easily identified by the corresponding primers marked on the cultivar identification diagram (CID). The results indicated that the CID strategy developed and employed in the apple cultivar identification could be vital in the utilization of DNA marker in other plants as well as the development of the apple industry.

分类号： S6

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