Comparison of six promoters for transient expression of luciferase reporter gene in cultured Bombyx mori cells (BmN)

文献类型： 外文期刊

第一作者： Huang, Yong

作者： Huang, Yong;Wang, Jian Ping;Huang, Yong;Song, Fei;Wang, Xin;Shen, Xing Jia

作者机构：

关键词： Bombyx mori cells;promoter;Luc reporter gene;expression activity

期刊名称：RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY （ 影响因子：0.718； 五年影响因子：0.727 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Luciferase (luc) reporter gene was used to construct recombinant plasmids containing six dif ferent promoters, which are the cytoplasmic actin3 promoter (A3), the fibroin heavy chain promoter (FibH), the fibroin light chain promoter (FibL), a glycoprotein (P25) promoter, Sericin1 promoter (Ser1) and the Bombyx mori nuclear polyhedrosis virus immediate (BmNPV) early protein promoter 1(IE1), respectively. These recombinant plasmids, which are pGL3A3luc, pGL3FibHluc, pGL3FibLluc, pGL3P25luc, pGL3Ser1luc and pGL3IE1luc had been constructed successfully by restriction enzyme digestion and PCR analysis, and then were transfected into BmN cells to measure the activity of the six promoters to drive luc reporter gene transient expression in cells. Transfection and transcriptional experiment indicated that except pGL3FibLluc, pGL3P25luc, pGL3Ser1luc, others three kinds of recombinant plasmids all transfected BmN cells obviously. The promoters of FibH, A3 and IE1 enhanced the transient expression activity of luc reporter gene in the BmN cells and their activity strengthened sequentially

分类号： Q

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