Expression and characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Pseudomonas

文献类型： 外文期刊

第一作者： Xiong, Fei

作者： Xiong, Fei;Shuai, Jian-Jun;Sun, Jing;Xiong, Ai-Sheng;Shuai, Jian-Jun;Jin, Xiao-Fen;Sun, Jing;Peng, Ri-He;Yao, Quan-Hong;Zhang, Jian

作者机构：

关键词： PsbphCI gene;2;3-dihydroxybiphenyl-1;2-dioxygenase;Synthesized gene;2;3-dihydroxybiphenyl;HPLC

期刊名称：MOLECULAR & CELLULAR TOXICOLOGY （ 影响因子：1.08； 五年影响因子：1.184 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The 2, 3-dihydroxybiphenyl-1,2-dioxygenase, which can degrade the 2,3-dihydroxybiphenyl, was encoded by a synthesized PsbphCI gene. The PsbphCI gene was transformed into Escherichia coli and the encoding protein purified, had a molecular mass of similar to 32 kDa as determined by SDS-PAGE. The optimum pH for the purified enzyme at 20A degrees C was 9.0, and the optimal temperature at pH 8.0 was 30A degrees C. Subsequently, the PsbphCI gene was transformed into Pseudomonas putida sp. to verify the degradation of 2,3-dihydroxybiphenyl by HPLC. The transgenic EG11 strain degraded 65.20% of the 2,3-DHBP after 2 minutes at 30A degrees C, while the wild-type EG11 strain degraded only 37.75%. This study provides guidance for the cultivation of bioengineered biphenyl/PCBs-degrading bacteria which can be applied to the biodegradation of environmental biphenyl/PCBs contamination.

分类号： Q7

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