Metagenomic cloning and characterization of Na+/H+ antiporter genes taken from sediments in Chaerhan Salt Lake in China

文献类型： 外文期刊

第一作者： Wang, Xiaoyu

作者： Wang, Xiaoyu;Chen, Sanfeng;Xu, Feng

作者机构：

关键词： Antiport activity;Metagenomic library;Na+/H+ antiporter

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A metagenomic library containing 8,000 clones was constructed by using genomic DNA obtained from Chaerhan Salt Lake in northwest China. Three Na+/H+ antiporters, C4-NhaG, C47-NhaG and C49-NhaG that grouped to the NhaG family, were screened and cloned from this metagenome by complementing Escherichia coli strain KNabc (Delta nhaA Delta nhaB Delta chaA) in medium containing 0.2 M NaCl. The three putative Na+/H+ antiporters were membrane proteins with 10, 11 and 11 transmembrane segments, respectively. They enabled E. coli KNabc to grow in medium containing 0.2-0.6 M Na+ or 7-14 mM Li+. Everted membrane vesicles prepared from E. coli KNabc cells carrying C49-NhaG exhibited Na+/H+ and Li+/H+ antiport activities.

分类号： Q7

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