Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products

文献类型： 外文期刊

第一作者： Ma, Fei

作者： Ma, Fei;Chen, Ran;Li, Peiwu;Zhang, Qi;Zhang, Wen;Hu, Xiaofeng;Ma, Fei;Chen, Ran;Li, Peiwu;Zhang, Qi;Zhang, Wen;Hu, Xiaofeng;Ma, Fei;Chen, Ran;Li, Peiwu;Zhang, Qi;Zhang, Wen;Ma, Fei;Chen, Ran;Li, Peiwu;Zhang, Wen;Hu, Xiaofeng

作者机构：

关键词： aflatoxins;immunoaffinity column;amino-silica gel;microparticle conjugate;agri-products;HPLC

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G1, B1, G2 and B2 were 0.03, 0.07, 0.05 and 0.09 μg·kg~(-1), and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg~(-1), respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg~(-1).

分类号： O62

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