An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice

文献类型： 外文期刊

第一作者： Liu, Zongliang

作者： Liu, Zongliang;Ji, Yanhong;Huang, Xiaohui;Fu, Yuguang;Cai, Xuepeng;Zhu, Qiyun;Wei, Jianzhong

作者机构：

关键词： Antibody-dependent enhancement;Duck Tembusu virus;Mouse model;Pathogenicity;Replication

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： Duck Tembusu virus (DTMUV) is a newly emerging infectious agent in China. Since 2010, this presumably arthropod-borne virus has caused severe disease in duck flocks. A cell-adapted DTMUV, designated AH10, has been developed. For this particular virus, replication, pathogenicity, and antibody-dependent enhancement (ADE) infection mediated by a specific antibody was investigated in mammalian cells and mice. The virus propagated in Vero or Hepa1-6 cells with a stable titer, and induced a visible cytopathic effect. Expression of pro- and anti-inflammatory cytokine genes in Hepa1-6 cells was elevated during early infection stages. AH10 was able to replicate in the brain, spleen, liver and kidneys of mice, and resulted in a systemic infection by day 5 when administered intracerebrally. When na?ve mice were first transfused intraperitoneally with virus-specific antisera, upon subsequent infection, ADE of virus symptoms was observed. Mice displayed clinical symptoms by day 3 post-infection; weight loss in the AH10-specific antibody-treated group was observed 2-3 days earlier than that in the control groups. These findings indicate that AH10 was able to replicate in the mouse model, and that anti-AH10 antibodies caused ADE. The molecular basis of pathogenicity and ADE in the mouse model should be investigated in the future.

分类号： R37

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