Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens

文献类型： 外文期刊

第一作者： Ding, Zhiping

作者： Ding, Zhiping;Liu, Zewen;Liu, Shuhua;Yang, Baojun;Zhang, Chengwei

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关键词： gene expression;gene expression regulation;gene silencing;RNA interference;gene targeting;knockout mutants;injection site;thorax;thoracic cavity;volume;Nilaparvata lugens;genes;calreticulin;central nervous system;plant pests;insect control.;nicotinic acetylcholine receptor;Internet resource.

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

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收录情况： SCI

摘要： RNA interference (RNAi) is a powerful strategy for gene function study in insects. Here, we described the development of a RNAi technique by microinjection of double-stranded RNA (dsRNA) in the brown planthopper Nilaparvata lugens. Based on the mortality and RNAi efficiency criteria, the conjunctive between prothorax and mesothorax was selected as the injection site and 50 nl as injection volume. Three genes with different expression patterns were selected to evaluate the RNAi efficiency. A comparable 40% decrease of gene expression was observed at the 4th day after injection for the ubiquitously expressed calreticulin and the gut specific cathepsin-B genes, but only 25% decrease at the 5th day for the central nervous system specific Nlo2 gene. Double injection could increase the RNAi efficiency, such as from 25% to 53% for Nlo2 gene. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in N. lugens.

分类号： Q5

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