Freeze-Drying of Glycoprotein GFPS1b from Grifola frondosa GF9801: Drying Kinetics, Functional, Conformational, and Topographical Changes
文献类型: 外文期刊
第一作者: Cui, Feng-Jie
作者: Cui, Feng-Jie;Zan, Xin-Yi;Sun, Wen-Jing;Huang, Da-Ming;Dong, Ying;Cui, Feng-Jie;Zhou, Yu-Guang;Yang, Yan;Sun, Wen-Jing
作者机构:
关键词: Anti-proliferative activity;Drying methods;Grifola frondosa;Physicochemical properties
期刊名称:DRYING TECHNOLOGY ( 影响因子:4.452; 五年影响因子:4.034 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: GFPSIb is an antitumor glycoprotein isolated and purified from the mycelia of Grifola frondosa GF9801. In the present study, freeze drying and hot air drying of GFPSIb were compared according to their drying kinetics, functional/conformational/topographical changes, and anti-proliferative activities for the first time. UV spectroscopy, FT-IR, SEM, and compositional analysis were conducted to evaluate the structural changes of GFPSIb after the above drying processes. Results showed that physicochemical and antiproliferative properties of GFPSIb differed from each other after the two drying treatments. The freeze-dried GFPSlb-F showed the significant advantages over hot-air-dried GFPSlb-A due to its higher product yield, higher protein content, total sugar content, uronic acid content, higher solubility, and anti-proliferative activity. This study revealed that different drying methods used for preparation of glycoprotein GFPSIb could significantly affect the physico-chemical properties and structure, which in turn adversely affect the biological activity of GFPSIb.
分类号: TQ424`TQ425
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