A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile cytoplasm in kenaf (Hibiscus cannabinus L.)

文献类型： 外文期刊

第一作者： Chen, Peng

作者： Chen, Peng;Liao, Xiaofang;Zhou, Bujin;Liao, Jian;Huang, Zhipeng;Kong, Xiangjun;Zhou, Ruiyang;Zhao, Yanhong

作者机构：

关键词： Kenaf (Hibiscus cannabinus L.);Cytoplasmic male sterility (CMS);atp9;Molecular marker;Quantitative PCR;Respiratory rate

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： mtDNA was isolated from cytoplasmic male sterility (CMS) line P3A and its maintainer P3B of kenaf (Hibiscus cannabinus L.). The atp9 gene and its two flanking sequences were obtained using homology cloning and high-efficiency thermal asymmetric interlaced PCR methods. The coding sequences showed only two base pairs difference between the CMS and its maintainer, and shared a homology of over 87 % with atp9 genes from other species in GenBank. However, when comparing the flanking sequences, a 47-bp deletion was characterized at the 3' flanking sequence of atp9 in the CMS line. Quantitative PCR analysis indicated that the expression level of atp9 in the CMS line was 0.937-fold that of its maintainer. Furthermore, the respiratory rate of anthers in the CMS line was markedly lower than that of its maintainer. The results indicated that the 47-bp deletion at the 3' flanking sequence of atp9 and/or down-regulated expression of the atp9 gene in the CMS line might be closely related to CMS in kenaf. To confirm whether the 47-bp deletion was specific to cytoplasm of male sterile lines, another 21 varieties were used for further analysis. The results showed that the 47-bp deletion was specific to male sterile cytoplasm (MSC) of kenaf. Based on these, a specific molecular marker was developed to distinguish the MSC from male fertile cytoplasm of kenaf.

分类号： Q94

  • 相关文献

[1]Mutation in the coding sequence of atp6 are associated with male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Liao, Xiaofang,Zhou, Bujin,Zhou, Yaoyao,Zhou, Ruiyang,Zhao, Hongtao,Zhou, Yaoyao.

[2]Expression of kenaf mitochondrial chimeric genes HM184 causes male sterility in transgenic tobacco plants. Liao, Xiaofang,Huang, Zhipeng,Chen, Peng,Zhou, Bujin,Liu, Dongmei,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong. 2015

[3]Reference genes selection for transcript normalization in kenaf (Hibiscus cannabinus L.) under salinity and drought stress. Niu, Xiaoping,Qi, Jianmin,Chen, Meixia,Tao, Aifen,Fang, Pingping,Xu, Jiantang,Onyedinma, Sandra A.,Chen, Meixia,Zhang, Gaoyang,Su, Jianguang. 2015

[4]Transcriptome Sequencing Analyses between the Cytoplasmic Male Sterile Line and Its Maintainer Line in Welsh Onion (Allium fistulosum L.). Liu, Qianchun,Wen, Changlong,Zhao, Hong,Wang, Jian,Wang, Yongqin,Lan, Yanping. 2016

[5]Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata). Wang, Qingbiao,Zhang, Yangyong,Fang, Zhiyuan,Liu, Yumei,Yang, Limei,Zhuang, Mu.

[6]Workable male sterility systems for hybrid rice: Genetics, biochemistry, molecular biology, and utilization. Huang, Jian-Zhong,Zhang, Hua-Li,Shu, Qing-Yao,E, Zhi-Guo. 2014

[7]Candidate Reference Genes Selection and Application for RT-qPCR Analysis in Kenaf with Cytoplasmic Male Sterility Background. Chen, Peng,Khan, Aziz,Chen, Lihong,Liao, Xiaofang,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong,Liu, Dongmei. 2017

[8]Effect of packaging films on quality of fresh-cut broccoli. Wang, Dan,Li, Xue,Ma, Yue,Zhao, Xiaoyan. 2014

[9]Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus. Yin, Fei,Sun, Peng,Gao, Quanxin,Shi, Zhaohong,Peng, Shiming,Gong, Qiyang,Li, Yanwei,Li, Anxing,Dan, Xueming. 2014

[10]NO3 (-)/NH4 (+) ratios affect plant growth, chlorophyll content, respiration rate, and morphological structure in Malus hupehensis seedlings. Dong, Yu,Zhao, Qian,Guan, Jun-feng,Dong, Yu,Zhi, Huan-huan,Zhao, Qian. 2015

[11]Research on respiration characteristics of fresh cut purple cabbage. Li, Zhu,Lu, Wang,Dan, Wang,Yue, Ma,Xiao-yan, Zhao,Li, Zhu,Lu, Wang,Dan, Wang,Yue, Ma,Xiao-yan, Zhao,Li, Zhu,Lu, Wang. 2016

[12]Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria. Zhang, Xiaoxia,Zhang, Ruijie,Gao, Jusheng,Wang, Xiucheng,Fan, Fenliang,Ma, Xiaotong,Zhang, Caiwen,Feng, Kai,Deng, Ye,Yin, Huaqun,Gao, Jusheng.

[13]Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an endogenous reference gene. Weng, H,Pan, AH,Yang, LT,Zhang, CM,Liu, ZL,Zhang, DB.

[14]Population dynamics and biocontrol efficacy of the nematophagous fungus Hirsutella minnesotensis in pot assay. Liu, Jianbin,Zhu, Weijing,Hamid, Mohammed Imran,Cheng, Xiaoli,Wang, Niuniu,Sun, Jingzu,Liu, Jianbin.

[15]Event-specific Qualitative And Quantitative Pcr Detection Of Genetically Modified Rapeseed Topas 19/2. Wu, Gang,Wu, Yuhua,Xiao, Ling,Lu, Changming.

[16]Cloning, Sequence and Expression Analysis of Gossypium barbadense L.pepc Gene. Yu Jiwen,Yu Shuxun,Zhai Honghong,Fan Shuli,Song Meizhen,Zhang Jinfa. 2011

[17]Novel zero-valent iron-assembled reactor for strengthening anammox performance under low temperature. Ren, Long-Fei,Lv, Lu,Zhang, Jian,Gao, Baoyu,Ni, Shou-Qing,Yang, Ning,Ren, Long-Fei,Zhou, Qingxin,Liu, Xiaoyong.

[18]Rice straw decomposition affects diversity and dynamics of soil fungal community, but not bacteria. Li, Peng,Pan, Aihu,Tang, Xueming,Li, Peng,Ming, Feng,Li, Yongchun,Zheng, Xianqing,Lv, Weiguang,Ding, Lina. 2018

[19]Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. Ding, JY,Jia, JW,Yang, LT,Wen, HB,Zhang, CM,Liu, WX,Zhang, DB. 2004

[20]Comparative proteomic analysis provides new insights into cadmium accumulation in rice grain under cadmium stress. Xue, Dawei,Deng, Xiangxiong,Zhang, Xiaoqin,Xu, Xiangbin,Qian, Qian,Xue, Dawei,Hu, Jiang,Zeng, Dali,Guo, Longbiao,Qian, Qian,Jiang, Hua,Wang, Hua. 2014

作者其他论文 更多>>

微信小程序