Histological and transcriptional characterization of a novel recessive genic male sterility mutant in sesame (Sesamum indicum L.)

文献类型： 外文期刊

第一作者： Wu, Kun

作者： Wu, Kun;Liu, Hongyan;Zuo, Yang;Yang, Minmin;Zhao, Yingzhong

作者机构：

关键词： Sesame;Recessive genic male sterility;Male gametogenesis;cDNA-AFLP;Real-time PCR

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recessive genic male sterility (RGMS) is an important approach to utilize heterosis, but the molecular mechanism of RGMS is not well understood in Sesamum indicum L. 95ms-5AB is a novel male sterility two-type line, which is controlled by a single RGMS gene. Histological observations revealed that abnormal inhibited male gametogenesis appeared during the mononuclear microspore stage. It was characterized by collapsed microspores, expanded vacuoles and the delayed degeneration of tapetum at the pollenmaturation stage. To elucidate the mechanism of this RGMS, the transcriptional profiles of the sterile flower buds in 95ms-5A and the fertile flower buds in 95ms-5B were investigated using the cDNA-AFLP (amplified fragment length polymorphism) technique. About 30 reproducible differentially expressed transcript-derived fragments (TDFs) were detected, including 27 up-regulated from fertile buds and three from sterile buds. The functional analysis of 27 successfully cloned TDFs indicated that the corresponding genes of 11 TDFs may participate in the processes of energy metabolism, signal transduction and plant cell development. The other 16 TDFs were homologous to the proteins with unknown functions or with no homology in GenBank. Real-time PCR analysisof 21 genes in fertile and sterile buds at four different stages was used to validate and further investigate their expression patterns identified by cDNA-AFLP. Our results suggested that several genes related to callose wall or tapetum degeneration were suppressed in 95ms-5A even before the microspore mother cell stage, which led to delayed tapetum degeneration and blocked male gametogenesis. Several RGMS-related novel genes, including one stamen specially expressed gene, had also been identified thatshowed significantly different expression level between fertile and sterile buds along sesame male gametogenesis.

分类号： Q94

  • 相关文献

[1]Pollen Semi-Sterility1 Encodes a Kinesin-1-Like Protein Important for Male Meiosis, Anther Dehiscence, and Fertility in Rice. Zhou, Shirong,Wang, Yang,Li, Wanchang,Zhao, Zhigang,Ren, Yulong,Wang, Yong,Jiang, Ling,Liu, Linglong,Wan, Jianmin,Zhou, Shirong,Gu, Suhai,Lin, Qibing,Wang, Dan,Su, Ning,Zhang, Xin,Cheng, Zhijun,Lei, Cailin,Wang, Jiulin,Guo, Xiuping,Wu, Fuqing,Wang, Haiyang,Wan, Jianmin,Ikehashi, Hiroshi.

[2]Characterization and genetic mapping of a novel recessive genic male sterile gene in sesame (Sesamum indicum L.). Zhao, Yingzhong,Wu, Jiangsheng,Liu, Kede,Zhao, Yingzhong,Yang, Minmin,Wu, Kun,Liu, Hongyan.

[3]Identification of Sesame Genomic Variations from Genome Comparison of Landrace and Variety. Wei, Xin,Zhu, Xiaodong,Yu, Jingyin,Wang, Linhai,Zhang, Yanxin,Li, Donghua,Zhou, Rong,Zhang, Xiurong. 2016

[4]Genome sequencing of the important oilseed crop Sesamum indicum L.. Zhang, Haiyang,Miao, Hongmei,Wang, Lei,Qu, Lingbo,Liu, Hongyan,Wang, Qiang,Yue, Meiwang. 2013

[5]Morpho-anatomical and physiological responses to waterlogging of sesame (Sesamum indicum L.). Wei, Wenliang,Li, Donghua,Wang, Linhai,Ding, Xia,Zhang, Yanxin,Gao, Yuan,Zhang, Xiurong. 2013

[6]Development of Simple Sequence Repeat (SSR) Markers of Sesame (Sesamum indicum) from a Genome Survey. Wei, Xin,Wang, Linhai,Zhang, Yanxin,Qi, Xiaoqiong,Wang, Xiaoling,Ding, Xia,Zhang, Jing,Zhang, Xiurong.

[7]Transcriptome comparison of resistant and susceptible sesame (Sesamum indicum L.) varieties inoculated with Fusarium oxysporum f.sp.sesami. Wei, Libin,Zhang, Haiyang,Duan, Yinghui,Li, Chun,Chang, Shuxian,Miao, Hongmei.

[8]Genetic analysis and molecular characterization of Chinese sesame (Sesamum indicum L.) cultivars using Insertion-Deletion (InDel) and Simple Sequence Repeat (SSR) markers. Wu, Kun,Yang, Minmin,Liu, Hongyan,Mei, Ju,Zhao, Yingzhong,Tao, Ye. 2014

[9]High-density genetic map construction and QTLs analysis of grain yield-related traits in Sesame (Sesamum indicum L.) based on RAD-Seq techonology. Wu, Kun,Liu, Hongyan,Yang, Minmin,Wu, Wenxiong,Zuo, Yang,Zhao, Yingzhong,Tao, Ye,Ma, Huihui. 2014

[10]Comprehensive Screening of Some West and Central African Sesame Genotypes for Drought Resistance Probing by Agromorphological, Physiological, Biochemical and Seed Quality Traits. Dossa, Komivi,Yehouessi, Louis W.,Cisse, Ndiaga,Dossa, Komivi,Liao, Boshou,Zhang, Xiurong,Dossa, Komivi,Diouf, Diaga,Likeng-Li-Ngue, Benoit C.,Bell, Joseph M.. 2017

[11]Nitrogen Recovery and Transport Efficiency of Winter Rapeseed and Residual Nitrogen Effect on Subsequent Sesame using N-15 Labelling Technique. Wang, Ruiqing,Zhang, Jing,Luo, Tao,Khan, Mohammad Nauman,Hu, Liyong,Wang, Ruiqing,Wei, Lingen. 2017

[12]Inheritance and molecular mapping of a novel dominant genic male-sterile gene in Sesamum indicum L.. Liu, Hongyan,Zhou, Xinan,Wu, Kun,Yang, Minmin,Zhao, Yingzhong.

[13]Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Liu, Hongyan,Zhou, Fang,Yang, Minmin,Zhou, Ting,Zhao, Yingzhong,Tan, Mingpu,Yu, Haijuan,Li, Liang. 2016

[14]Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses. Li, Donghua,Liu, Pan,Yu, Jingyin,Wang, Linhai,Dossa, Komivi,Zhang, Yanxin,Zhou, Rong,Wei, Xin,Zhang, Xiurong,Dossa, Komivi,Wei, Xin. 2017

[15]Development, inheritance and breeding potential of a recessive genic male sterile line D248A in Sesame (Sesamum indicum L.). Liu, Hongyan,Yang, Minmin,Wu, Kun,Zhou, Xinan,Zhao, Yingzhong. 2013

[16]Genome-wide identification and analysis of the MADS-box gene family in sesame. Wei, Xin,Wang, Linhai,Yu, Jingyin,Zhang, Yanxin,Li, Donghua,Zhang, Xiurong.

[17]Development of SNP and InDel markers via de novo transcriptome assembly in Sesamum indicum L.. Wei, Libin,Miao, Hongmei,Li, Chun,Duan, Yinghui,Niu, Jiaojiao,Zhang, Tide,Zhang, Haiyang,Zhao, Qiongyi.

[18]Genetic diversity assessment of sesame core collection in China by phenotype and molecular markers and extraction of a mini-core collection. Zhang, Yanxin,Zhang, Xiurong,Che, Zhuo,Wang, Linhai,Wei, Wenliang,Li, Donghua,Che, Zhuo. 2012

[19]Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Zhang, Yan-xin,Zhang, Xiu-rong,Hua, Wei,Wang, Lin-han,Che, Zhuo. 2010

[20]A comparison of methods for drying seeds: vacuum freeze-drier versus silica gel. Hu, XR,Zhang, YL,Hu, CL,Tao, M,Chen, SP. 1998

作者其他论文 更多>>

微信小程序