Determination of Cattle Foot-and-Mouth Disease Virus by Micro-ELISA Method

文献类型: 外文期刊

第一作者: Dong, Yiyang

作者: Dong, Yiyang;Xu, Yan;Xu, Yan;Mawatari, Kazuma;Kitamori, Takehiko;Liu, Zaixin;Fu, Yuanfang;Ohashi, Toshinori;Mawatari, Kazuma;Kitamori, Takehiko

作者机构:

关键词: Microchip;micro-ELISA;foot-and-mouth disease virus

期刊名称:ANALYTICAL SCIENCES ( 影响因子:2.081; 五年影响因子:1.676 )

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年卷期:

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收录情况: SCI

摘要: The development of foot-and-mouth disease virus (FMDV) detection methods is crucial for animal food security, tackling regional FMDV epidemic, and global FMDV prognostic control. For these purposes, a fast and sensitive analysis method is required. In this study, we developed a microchip-based ELISA (enzyme-Unked immunosorbent assay), micro-ELISA, to realize FMDV detection. Nickel(II) chelating chemistry was utilized to immobilize recombinant protein (antigen) on polystyrene micro-beads in order to determine FMDV antibodies in cattle serum samples. In addition, reaction protocol and conditions were investigated. As a result, the FMDV detection was successfully demonstrated with only a 10-μL sample volume in 25-minute assay time. Analytical sensitivity was evaluated by a maximum nominal positiveness percentage value (NPPV) of 303 and a dilution factor of 32x. The method's inter-run and intra-run CV (coefficients of variance) values were 15.5 and 17.1%, respectively, which were fully compatible with the OIE (World Organization for Animal Health) principle of validation of diagnosis assays for infectious diseases. The developed method should become a powerful tool for determining other animal contagious diseases and/or zoonosis.

分类号: O65

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