Self-assembly of virus-like particles of canine parvovirus capsid protein expressed from Escherichia coli and application as virus-like particle vaccine

文献类型： 外文期刊

第一作者： Xu, Jin

作者： Xu, Jin;Guo, Hui-Chen;Wei, Yan-Quan;Dong, Hu;Han, Shi-Chong;Ao, Da;Sun, De-Hui;Wang, Hai-Ming;Sun, Shi-Qi;Xu, Jin;Guo, Hui-Chen;Wei, Yan-Quan;Dong, Hu;Han, Shi-Chong;Ao, Da;Sun, De-Hui;Wang, Hai-Ming;Sun, Shi-Qi;Xu, Jin;Ao, Da;Cao, Sui-Zhong

作者机构：

关键词： Canine parvovirus;Prokaryotic expression;Virus-like particles;Immunogenicity;Capsid protein

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Canine parvovirus disease is an acute infectious disease caused by canine parvovirus (CPV). Current commercial vaccines are mainly attenuated and inactivated; as such, problems concerning safety may occur. To resolve this problem, researchers developed virus-like particles (VLPs) as biological nanoparticles resembling natural virions and showing high bio-safety. This property allows the use of VLPs for vaccine development and mechanism studies of viral infections. Tissue-specific drug delivery also employs VLPs as biological nanomaterials. Therefore, VLPs derived from CPV have a great potential in medicine and diagnostics. In this study, small ubiquitin-like modifier (SUMO) fusion motif was utilized to express a whole, naturalVP2 protein of CPVin Escherichia coli. After the cleavage of the fusion motif, the CPV VP2 protein has self-assembled into VLPs. The VLPs had a size and shape that resembled the authentic virus capsid. However, the self-assembly efficiency of VLPs can be affected by different pH levels and ionic strengths. The mice vaccinated subcutaneously with CPV VLPs and CPV-specific immune responses were comparedwith those immunizedwith the natural virus. This result showed that VLPs can effectively induce anti-CPV specific antibody and lymphocyte proliferation as a whole virus. This result further suggested that the antigen epitope of CPV was correctly present on VLPs, thereby showing the potential application of a VLP-based CPV vaccine.

分类号： Q939.9`Q81

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