Development of a direct competitive chemiluminescent ELISA for the detection of nitrofurantoin metabolite 1-amino-hydantoin in fish and honey

文献类型： 外文期刊

第一作者： Wang, Quan

作者： Wang, Quan;Liu, Ying-Chun;Chen, Yong-Jun;Jiang, Wei;Shi, Jin-Lei;Xiao, Yan;Zhang, Meng

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期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

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收录情况： SCI

摘要： A direct competitive enzyme-linked immunosorbent assay (ELISA) with chemiluminescent (dcCLELISA) detection for 1-amino-hydantoin (AHD) was developed in this study. AHD was derivatised with 4-carboxybenzaldehyde to produce l-[(4-carbo-benzylidene)-aminoHmidazolidin-2,4-dione (CPAHD) Monoclonal antibodies (MAb) against AHD were prepared through immunization of BALB/c mice with synthesized CPAHD-Jeffamine-BSA as an antigen. Luminol, p-iodophenol, and urea peroxide mixture solution served as the substrate in CLEUSA. The specificity of the MAb, estimated as the cross-reactivity values from the dcCLEIUSA assay for l-[(4-nitro-benzylidene)-amino]-imidazolidin-2,4-dione (NPAHD) and CPAHD, was 100% and 39.67%, respectively. All other compounds showed less than 0.01%, The sensitivity of the antibody, estimated as the IC_(50) value, was 0.60 ug L~(-1). The limits of detection for dcCLELISA in fish and honey samples were 0.1 and 0.28 \xg kg~(-1), respectively, and the mean recovery values ranged from 83.6% to 94.7% for fortified samples at levels of 0.25-10 ug kg~(-1) with coefficient of variation values below 15%. Finally, dcCLELISA was compared to a commercial kit in the detection of AHD in spiked fish and honey samples. The immunoassay method described here showed a broad detection range and high sensitivity. It could be used for high-throughput monitoring of AHD in fish and honey samples and possibly other types of food.

分类号： O65

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