Purification and characterization of a laccase from Coprinopsis cinerea in Pichia pastoris

文献类型： 外文期刊

第一作者： Wang, Bo

作者： Wang, Bo;Wang, Lijuan;Lin, Yaqiu;Han, Jing;Gao, Jianjie;Tian, Yongsheng;Zhao, Wei;Peng, Rihe;Yao, Quanhong;Wang, Bo;Wang, Lijuan;Han, Jing;Gao, Jianjie;Tian, Yongsheng;Zhao, Wei;Peng, Rihe;Yao, Quanhong;Lin, Yaqiu;Han, Qing;Han, Qing

作者机构：

关键词： Laccase;Pichia pastoris;Coprinopsis cinerea;Purification;Biochemical properties;Dye decolorization

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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年卷期：

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收录情况： SCI

摘要： A modified laccase gene, CcLCC6, from Coprinopsis cinerea was chemically synthesized according to the yeast codon bias and expressed in Pichia pastoris. The main properties of laccase, effects of ions and inhibitors, and optimal condition for decolouring malachite green (MG) were investigated in this study. The optimal pH level and temperature of laccase are 3.0 and 40iu pC, respectively. The metal ions Mnpo, Znpo, Fepd and Alpd could inhibit laccase activity, as well as 1iu mM of sodium dodecyl sulphate and sodium thiosulphate. 2,2o-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), as a mediator, was necessary in decolorizing MG. The optimal pH and temperature for MG decolorization were 3.0 and 50iu pC, respectively. Approximately 0.02iu oM recombinant laccase could effectively decolour 0.05iu mM of MG in 1iu h. CcLCC6I could inhibit the toxicity of MG to P. pastoris. This is the first report on the successful expression in P. pastoris of CcLCC6I and its enzymatic property. Laccase can also be considered as a candidate for treating industrial effluent containing MG.

分类号： Q93

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