文献类型: 外文期刊
第一作者: Wang, Chunmei
作者: Wang, Chunmei;Chen, Fangzhou;Hu, Han;Li, Wentao;Chen, Pin;Liu, Yingyu;Ku, Xugang;He, Qigai;Chen, Huanchun;Wang, Chunmei;Xue, Feiqun;Wang, Yang
作者机构:
关键词: Antimicrobial peptides;Cecropin B;DNA microarray;Haemophilus parasuis;Transcriptional profiling
期刊名称:JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:0.803; 五年影响因子:1.609 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Synthetically designed antimicrobial peptides (AMPs) present the potential of replacing antibiotics in the treatment of bacterial infections. However, microbial resistance to AMPs has been reported and little is known regarding the underlying mechanism of such resistance. The naturally occurring AMP cecropin B (CB) disrupts the anionic cell membranes of Gram-negative bacteria. In this study, CB resistance (CBR) was induced in Haemophilus parasuis SH0165 by exposing it to a series of CB concentrations. The CB-resistant H. parasuis strains CBR30 and CBR30-50 were obtained. The growth curves of SH0165 and CBR30 showed that CBR30 displayed lower growth rates than SH0165. The result of transmission electron microscopy showed cell membranes of the CB-resistant CBR30 and CBR30-50 were smoother than SH0165. Microarrays detected 257 upregulated and 254 downregulated genes covering 20 clusters of orthologous groups (COGs) of the CB-resistant CBR30 compared with SH0165 (>1.5-fold change, p < 0.05). Sixty genes were affected in CBR30-50 covering 18 COGs, with 28 upregulated and 32 downregulated genes. Under the COG function classification, the majority of affected genes in the CB-resistant CBR30 and CBR30-50 belong to the category of inorganic ion transport, amino acid transport, and metabolism. The microarray results were validated by real-time quantitative reverse transcription PCR. This study may provide useful guidance for understanding the molecular mechanism underlying H. parasuis resistance to CB.
分类号: Q93`Q81
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