Influence of Gamma Irradiation and Heat Treatment on the Immunogenicity of Five Epitopes of Pen a1

文献类型： 外文期刊

第一作者： Mou, Hui

作者： Mou, Hui;Gao, Mei-xu;Zhao, Jie;Zhao, Xin;Wang, Zhi-dong;Pan, Jia-rong

作者机构：

关键词： Pen a1;epitopes;gamma irradiation;heat;immunoreactivity

期刊名称：FOOD SCIENCE AND TECHNOLOGY RESEARCH （ 影响因子：0.668； 五年影响因子：0.895 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Background: Pen a1 is a major allergen in shrimp. It is a 284 amino acid protein member of the tropomyosin family with a molecular weight of 36 kDa. Irradiation and heat treatments have been previously shown to reduce the immunoreactivity of Pen a1, but since anaphylaxis is not induced by the whole protein but by epitopes within the protein, studying of the influence of these treatments on the epitopes of Pen a1 is helpful for understanding the sensitization mechanism. In this study, the immunoreactivity of the five epitopes of Pen a1 exposed to heat and irradiation treatments was detected by Ci-ELISA and western blot, using specific anti-sera generated from 5 synthesized epitope peptides of Pen a1. We examined the function of each epitope in the sensitization process by analyzing the variations of their immunoreactivity following irradiation, heat treatment, or a combination of both. RESULTS: Pen a1 was treated by gamma irradiation, heat at 100℃ or a combination of irradiation and heat. The IC_(50) value for each of the five epitopes was detected. The IC_(50) value of epitopes 1, 2, and 4 decreased under all treatments, especially epitope 4; its IC_(50) value decreased sharply from 15.88 to 0.11 follwing 7 kGy irradiation and to 0.03 - 0.04 under 10 kGy or 7 kGy irradiation and heat. The IC_(50) value of epitope 3 increased from 1.49 to 5.32 under 7 kGy irradiation and to 10.03 under 7kGy and heat, but mildly decreased after 10 kGy irradiation. In contrast, the IC_(50) value of epitope 5 hardly changed following any treatments. CONCLUSION: The variation in the IC_(50) values of epitope 3 was consistent with the IC_(50) values measured from the whole Pen a1 protein, while epitope 5 was stable following irradiation and heat treatments. Since the variation of immunoreactivity of epitope 3 was consistent with Pen a1, this suggests that epitope 3 is the determinative epitope in the allergenicity of Pen a1; epitope 5 was stable with all treatment conditions, suggesting that it could be used in immunoassay kits for the detection of treated shrimp.

分类号： TS2

  • 相关文献

[1]Critical Amino Acids Analysis of Epitopes in Pen a1. Mou Hui,Gao Mei-Xu,Wang Zhi-Dong,Zhao Jie,Li Shu-Jin,Zhao Xin,Li Shu-Rong,Pan Jia-Rong,Zhi Yu-Xiang. 2014

[2]Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus. Zhang, Lihong,He, Zhi,Wu, Yangsheng,Zhang, Weimin,Xie, Jun,Wang, Taixin.

[3]OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis. Jin, Xiao-Fen,Xiong, Ai-Sheng,Peng, Ri-He,Liu, Jin-Ge,Gao, Feng,Yao, Quan-Hong,Chen, Jian-Min.

[4]Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice. Ye ShuiFeng,Yu ShunWu,Shu LieBo,Wu JinHong,Luo LiJun,Ye ShuiFeng,Wu AiZhong,Wu AiZhong. 2012

[5]High temperature effects on flavones accumulation and antioxidant system in Scutellaria baicalensis Georgi cells. Yuan, Yuan,Luo, Yujian,Huang, Luqi,Chen, Shunqin,Yang, Zhaochun,Qin, Shuangshuang,Liu, Yunjun. 2011

[6]Strategic crossing of biomass and harvest index-source and sink-achieves genetic gains in wheat. Reynolds, Matthew P.,Pask, Alistair J. D.,Sonder, Kai,Sukumaran, Sivakumar,Molero, Gemma,Saint Pierre, Carolina,Payne, Thomas,Singh, Ravi P.,Braun, Hans J.,Hoppitt, William J. E.,Gonzalez, Fernanda G.,Terrile, Ignacio I.,Barma, Naresh C. D.,Hakim, Abdul,He, Zhonghu,Fan, Zheru,Novoselovic, Dario,Maghraby, Maher,Gad, Khaled I. M.,Galal, ElHusseiny G.,Hagras, Adel,Mohamed, Mohamed M.,Morad, Abdul Fatah A.,Kumar, Uttam,Singh, Gyanendra P.,Naik, Rudra,Kalappanavar, Ishwar K.,Biradar, Suma,Prasad, Sakuru V. Sai,Chatrath, Ravish,Sharma, Indu,Panchabhai, Kishor,Sohu, Virinder S.,Mavi, Gurvinder S.,Mishra, Vinod K.,Balasubramaniam, Arun,Jalal-Kamali, Mohammad R.,Khodarahmi, Manoochehr,Dastfal, Manoochehr,Tabib-Ghaffari, Seyed M.,Jafarby, Jabbar,Nikzad, Ahmad R.,Moghaddam, Hossein Akbari,Ghojogh, Hassan,Mehraban, Asghar,Solis-Moya, Ernesto,Camacho-Casas, Miguel A.,Figueroa-Lopez, Pedro,Ireta-Moreno, Javier,Alvarado-Padilla, Jorge I.,Borbon-Gracia, Alberto,Torres, Arace.

[7]Effects of gamma-irradiation before and after cooking on bacterial population and sensory quality of Dakgalbi. Yoon, Young Min,Park, Jong-Heum,Lee, Ji-Hye,Park, Jae-Nam,Park, Jin-Kyu,Sung, Nak-Yun,Song, Beom-Seok,Kim, Jae-Hun,Lee, Ju-Woon,Yoon, Young Min,Lee, Ji-Hye,Yook, Hong-Sun,Yoon, Yohan,Gao, Meixu. 2012

[8]Analysis and identification of irradiated Spirulina powder by a three-step infrared macro-fingerprint spectroscopy. Liu, Hai Jing,Wang, Feng,Li, Wei Ming,Ha, Yi Ming,Liu, Hai Jing,Wang, Feng,Li, Wei Ming,Ha, Yi Ming,Xu, Chang Hua,Zhou, Qun,Sun, Su Qin. 2013

[9]Detection of gamma-irradiated peanuts by ESR spectroscopy and GC analysis of hydrocarbons. Li, Wei Ming,Li, An,Ha, Yi Ming,Wang, Feng,Zhang, Yan Li. 2011

[10]Effect of high-dose irradiation and autoclave treatment on microbial safety and quality of ready-to-eat Bulgogi sauce. Park, Jin-Gyu,Song, Beom-Seok,Kim, Jae-Hun,Han, In-Jun,Lee, Ju-Woon,Park, Jin-Gyu,Yoon, Yohan,Chung, Hyung-Wook,Kim, Eun-Jeong,Gao, Meixu. 2012

[11]2-Dodecylcyclobutanone as an irradiation dose indicator to identify and estimate original dose in irradiated ground beef. Zhao, Yueliang,Wang, Feng,Li, Weiming,Li, An,Ha, Yiming,Zhao, Yueliang,Wang, Feng,Li, Weiming,Li, An,Ha, Yiming. 2012

[12]Analysis of degradation products and structural characterization of giant reed and Chinese silvergrass pretreated by Co-60-gamma irradiation. Li, Qing-ming,Li, Xia-jie,Jiang, Yi-le,Tan, Xing-he,Li, Qing-ming,Hu, Qiu-long,Wang, Ke-qin,Su, Xiao-jun,Su, Xiao-jun,Xiong, Xing-yao.

[13]Comparison of gamma irradiation and steam explosion pretreatment for ethanol production from agricultural residues. Chen, Jing-ping,Chen, Liang,Xiong, Xing-yao,Su, Xiaojun,Liu, Yun. 2012

[14]Influence of irradiation dose on free volume and microstructure of SIB biblock copolymer study by PALS and FT-IR. Jiang Zhong-Ying,Yu Wei-Zhong,Zhao Yong-Fu,Jiang Xi-Qun,Xia Yuan-Fu. 2006

[15]Gamma irradiation-induced sulfadiazine degradation and its removal mechanisms. Guo, Zhaobing,Zhou, Fei,Zhang, Chaozhi,Liu, Fengling,Bao, Chunxiao,Lin, Mingyue,Guo, Zhaobing,Zhou, Fei,Zhang, Chaozhi,Liu, Fengling,Bao, Chunxiao,Lin, Mingyue,Zhao, Yongfu.

[16]Decomposition of dexamethasone by gamma irradiation: Kinetics, degradation mechanisms and impact on algae growth. Guo, Zhaobing,Guo, Anke,Rui, Maoling,Zhang, Haixiao,Zhu, Shengnan,Guo, Zhaobing,Guo, Anke,Rui, Maoling,Zhang, Haixiao,Zhu, Shengnan,Guo, Qingjun,Zhao, Yongfu.

[17]Radiolytic decomposition of ciprofloxacin using gamma irradiation in aqueous solution. Guo, Zhaobing,Zhu, Shengnan,Cao, Hui,Liu, Fengling,Guo, Zhaobing,Zhu, Shengnan,Cao, Hui,Liu, Fengling,Zhao, Yongfu.

[18]Gamma irradiation-induced decomposition of sulfamethoxazole in aqueous solution: the influence of additives, biological inhibitory, and degradation mechanisms. Wang, Jinjin,Guo, Ziyan,Shen, Xiaoyu,Zhu, Shengnan,Guo, Zhaobing,Wang, Jinjin,Guo, Ziyan,Shen, Xiaoyu,Zhu, Shengnan,Guo, Zhaobing,Wang, Jinjin,Guo, Ziyan,Shen, Xiaoyu,Zhu, Shengnan,Guo, Zhaobing,Guo, Qingjun,Zhao, Yongfu.

[19]Analysis of murine B-cell epitopes on bluetongue virus 12 nonstructural protein 1. Wang HaiXiu,Sun EnCheng,Xu QingYuan,Yang Tao,Zhang Qin,Feng YuFei,Li JunPing,Lv Shuang,Sun Liang,Sun Jing,Wu DongLai.

[20]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

作者其他论文 更多>>

微信小程序