Megasporogenesis, megagametogenesis, and induction of 2n eggs with colchicine in poplar section Aigeiros

文献类型： 外文期刊

第一作者： Xi, Xiaojun

作者： Xi, Xiaojun;Guo, Liqin;Xu, Wenting;Zhang, Jinfeng;Li, Bailian;Xi, Xiaojun;Li, Bailian

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关键词： Populus;2n eggs;megasporogenesis;megametogenesis;triploid breeding

期刊名称：SCANDINAVIAN JOURNAL OF FOREST RESEARCH （ 影响因子：2.103； 五年影响因子：2.003 ）

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收录情况： SCI

摘要： Triploid breeding through 2n eggs induction has become one of the most powerful approaches for the improvement of the genus Populus. To enhance the efficiency of triploid production in section Aigeiros, female buds (catkins) of P. x euramericana (Dode) Guinier at different developmental stages were exposed to colchicine for 2n eggs induction. The relationship between flower bud morphological characteristics (or time after pollination) and female meiotic stage (or embryo sac development) was established to guide colchicine treatments. In the resulting progeny, three triploid seedlings were obtained via embryo rescue and identified by flow cytometric analyses. Cytological observation revealed that the leptotene to pachytene stage of meiosis and 12 h after pollination might be the suitable period for 2n eggs induction with colchicine in P. x euramericana. Results and protocols related to 2n eggs induction, effective methodologies for embryo rescue, and ploidy evaluation in this study might be applicable in polyploidy breeding in section Aigeiros.

分类号： S7

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