Threonine deaminase MoIlv1 is important for conidiogenesis and pathogenesis in the rice blast fungus Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Du, Yan

作者： Du, Yan;Hong, Li;Tang, Wei;Li, Lianwei;Wang, Xiaoli;Ma, Hongyu;Zhang, Haifeng;Zheng, Xiaobo;Zhang, Zhengguang;Du, Yan;Wang, Zhengyi

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关键词： Magnaporthe oryzae;Threonine deaminase;MoIlv1;Conidiogenesis;Pathogenesis

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.495； 五年影响因子：3.831 ）

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收录情况： SCI

摘要： Threonine deaminase is the first critical enzyme in the biosynthesis of branched-chain amino acids (BCAAs), which catalyzes threonine into NH2 and ketobutyrate acid. Previously, we identified and characterized two acetolactate synthases MoIlv2 and MoIlv6 that are involved in the second step of BCAA bio-synthesis. Deletion of MoILV2 and MoILV6 resulted in auxotrophy for leucine, isoleucine, and valine and defects in conidiation, appressorial penetration, and pathogenicity. Here, we identified a threonine dehydratase, named MoIlv1, from Magnaporthe oryzae. MoIlv1 is a homolog of Saccharomyces cerevisiae Ilv1p, which has an important role in the biosynthesis of isoleucine. To characterize the function of MoIlv1, a Delta MoIlv1 knock-out mutant was generated and analyzed. Disruption of MoILV1 resulted in abnormal conidial morphology, reduced conidiation, limited appressorium-mediated penetration, and attenuated virulence on both barley and rice seedlings. Further analysis by domain-specific deletion revealed that the PALP domain is indispensable for MoIlv1 function. Our study indicates that MoIlv1 is a protein involved in isoleucine biosynthesis that underlies the complex process governing morphogenesis, appressorium formation, invasive hyphae growth, and pathogenicity. (C) 2014 Elsevier Inc. All rights reserved.

分类号： Q939.5

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