Characterization of the chaperonin GroEL in Mycoplasma gallisepticum

文献类型： 外文期刊

第一作者： Tan, Lei

作者： Tan, Lei;Hu, Meirong;Yu, Shengqing;Wang, Xin;Lu, Feng;Liu, Fang;Qiu, Xusheng;Song, Cuiping;Sun, Yingjie;Ding, Chan

作者机构：

关键词： Mycoplasma gallisepticum;Two-dimensional gel electrophoresis;GroEL;ATPase activity;Bactericidal assay

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.552； 五年影响因子：2.475 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mycoplasma gallisepticum (MG) is a common and widespread cause of chronic respiratory disease in poultry. In this study, antigenic proteins were identified from MG membrane using two-dimensional gel electrophoresis (2-DE) analysis followed by Western blot and matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), including translation elongation factor Tu, dihydrolipoamide acetyltransferase (E2) component of pyruvate dehydrogenase complex, trigger factor, chaperone protein DnaK, heat shock protein GroEL and so on. Furthermore, recombinant MG GroEL protein was successfully expressed in E. coli BL21 (DE3) with pET-28a (+) vector and found to possess ATPase activity and contributed to the refolding of recombinant MG PrpC protein. Complement-dependent bactericidal assay indicated that the rabbit antisera against MG rGroEL had satisfactory bactericidal effect, which is similar to the chicken antisera induced by MG-inactivated vaccine, suggesting MG GroEL is a protective antigen, could be used as a novel vaccine candidate. This study is the first report of the biological characterization of chaperone GroEL protein in MG.

分类号： Q93

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