Simultaneous Determination of Malachite Green, Diethylstilbestrol, Medroxyprogesterone, and 3-Amino-2-Oxazolidone in Synbranchoid Eels by a Protein Microarray Method

文献类型： 外文期刊

第一作者： Liu, Ying-Chun

作者： Liu, Ying-Chun;Wang, Quan;Jiang, Wei;Chen, Yong-Jun;Zhao, Xin-Yu;Jing, Zhen-Yu;Zhang, Meng

作者机构：

关键词： Protein microarray;Drug residue;Malachite green;Diethylstilbestrol;Medroxyprogesterone;3-amino-2-oxazolidone

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

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收录情况： SCI

摘要： Protein chips have emerged as a useful approach for a wide variety of applications, including the identification of small molecule targets. In this study, a protein microarray method was developed for the simultaneous detection of the following four veterinary drug residues in synbranchoid eels: leucomalachite green (LMG), diethylstilbestrol (DES), medroxyprogesterone (MPA), and 3-amino-2-oxazolidone (AOZ). The analytes were coupled to ovalbumin (OVA) before immobilization on the surface of modified glass slides. After immobilization, a mixture of antibodies to the four analytes and either standard solutions containing the analytes or samples were added to the array reaction area. Then, Cy3-labeled goat anti-rabbit IgG was added and analyte residues were detected quantitatively. The results indicated that the 50 % inhibitory concentrations of DES, MPA, LMG, and AOZ were 2.0, 1.98, 1.65, and 0.355 mu g/L, respectively. The limit of detection of DES, MPA, LMG, and AOZ in synbranchoid eels were 0.325, 0.37, 0.271, and 0.103 mu g/kg, respectively, and recovery rates ranged from 60.7 to 98.6 % for fortified samples at levels of 2, 5, and 10 mu g/kg with coefficient of variation values < 15 %. This protein microarray method was compared with commercially available kits for these four analytes; both methods yielded similar results. These results indicate that the protein microarray method is suitable for the detection of DES, MPA, LMG, and AOZ, as well as other potential drug residues in animal tissues.

分类号： TS2

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