Simultaneous Determination of Malachite Green, Diethylstilbestrol, Medroxyprogesterone, and 3-Amino-2-Oxazolidone in Synbranchoid Eels by a Protein Microarray Method

文献类型： 外文期刊

第一作者： Liu, Ying-Chun

作者： Liu, Ying-Chun;Wang, Quan;Jiang, Wei;Chen, Yong-Jun;Zhao, Xin-Yu;Jing, Zhen-Yu;Zhang, Meng

作者机构：

关键词： Protein microarray;Drug residue;Malachite green;Diethylstilbestrol;Medroxyprogesterone;3-amino-2-oxazolidone

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Protein chips have emerged as a useful approach for a wide variety of applications, including the identification of small molecule targets. In this study, a protein microarray method was developed for the simultaneous detection of the following four veterinary drug residues in synbranchoid eels: leucomalachite green (LMG), diethylstilbestrol (DES), medroxyprogesterone (MPA), and 3-amino-2-oxazolidone (AOZ). The analytes were coupled to ovalbumin (OVA) before immobilization on the surface of modified glass slides. After immobilization, a mixture of antibodies to the four analytes and either standard solutions containing the analytes or samples were added to the array reaction area. Then, Cy3-labeled goat anti-rabbit IgG was added and analyte residues were detected quantitatively. The results indicated that the 50 % inhibitory concentrations of DES, MPA, LMG, and AOZ were 2.0, 1.98, 1.65, and 0.355 mu g/L, respectively. The limit of detection of DES, MPA, LMG, and AOZ in synbranchoid eels were 0.325, 0.37, 0.271, and 0.103 mu g/kg, respectively, and recovery rates ranged from 60.7 to 98.6 % for fortified samples at levels of 2, 5, and 10 mu g/kg with coefficient of variation values < 15 %. This protein microarray method was compared with commercially available kits for these four analytes; both methods yielded similar results. These results indicate that the protein microarray method is suitable for the detection of DES, MPA, LMG, and AOZ, as well as other potential drug residues in animal tissues.

分类号： TS2

  • 相关文献

[1]Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples. Li WeiHua,Liu Wei,Yin WeiWei,Xu Jing,Liu JinTing,Meng Meng,Xue HuYin,Xi RiMo,Meng Meng,Xue HuYin,Xi RiMo,He FangYang,Wan YuPing,Feng CaiWei,Wang ShanLiang,Lu Xiao. 2011

[2]Simultaneous Detection of Diethylstilbestrol and Malachite Green Using Conductive Carbon Black Paste Electrode. Qu, Keming,Zhang, Xuzhi,Li, Meng,Cui, Zhengguo,Zhang, Yan,Chen, Bijuan,Ma, Shaosai,Lv, Zhilin,Kong, Qing,Li, Meng. 2012

[3]Smartphone-based visualized microarray detection for multiplexed harmful substances in milk. Li, Zhoumin,Li, Zhonghui,Zhao, Dingyi,Xu, Danke,Li, Zhoumin,Wen, Fang,Jiang, Jindou,Zhao, Dingyi.

[4]Detection of antibodies against avian influenza virus by protein microarray using nucleoprotein expressed in insect cells. Zhao, Yuhui,Li, Guangxing,Wang, Xiurong,Chen, Pucheng,Zeng, Xianying,Bao, Hongmei,Wang, Yunhe,Xu, Xiaolong,Jiang, Yongping,Chen, Hualan.

[5]Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds. Zhang, Taichang,Meng, Meng,Xi, Rimo,Yin, Weiwei,Liu, Jinting,Li, Weihua,Liu, Wei,He, Fangyang,Wan, Yuping,Feng, Caiwei,Wang, Shanliang,Lu, Xiao.

[6]Enhanced effects by mixtures of three estrogenic compounds at environmentally relevant levels on development of Chinese rare minnow (Gobiocypris rarus). Yang, Fangxing,Jin, Shiwei,Jin, Shiwei,Hui, Yang,Xu, Ying,Liao, Tao,Wen, Sheng.

[7]Separation of hexestrol, diethylstilbestrol and dienestrol in milk by high performance liquid chromatography. Liu Hong-Cheng,Zou Yan-Hong,Li Qi-Wan,Zhu, Er. 2008

[8]Portable SERS sensor for malachite green and other small dye molecules. Qiu, Suyan,Qiu, Suyan,Zhao, Fusheng,Li, Jingting,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan. 2017

[9]Persistence of malachite green and leucomalachite green in perch (Lateolabrax japonicus). Tan Zhijun,Xing Lihong,Guo Mengmeng,Wang Hongyan,Jiang Yanhua,Li Zhaoxin,Zhai Yuxiu,Tan Zhijun,Xing Lihong,Guo Mengmeng,Wang Hongyan,Jiang Yanhua,Li Zhaoxin,Zhai Yuxiu,Wang Hongyan. 2011

[10]Determination of the malachite green in sediment by high performance liquid chromatography with fluorescence detection. Deng, Jianchao,Yang, Xianqing,Li, Laihao,Cen, Jianwei,Hao, Shuxian,Wei, Ya,Xin, Shaoping. 2013

[11]Nanoporous Gold Disks Functionalized with Stabilized G-Quadruplex Moieties for Sensing Small Molecules. Qiu, Suyan,Zhao, Fusheng,Zenasni, Oussama,Li, Jingting,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan,Qiu, Suyan. 2016

[12]A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment. Zhu, Dan,Pang, Xiumei,Liu, Yue,Wang, Xue,Chen, Gang,Li, Qiangqiang.

[13]Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry. Zhu, Dan,Li, Qiangqiang,Chen, Gang,Honeychurch, Kevin C.,Piano, Martina.

[14]Heterologous expression and characterization of laccase 2 from Coprinopsis cinerea capable of decolourizing different recalcitrant dyes. Tian, Yong-Sheng,Xu, Hu,Peng, Ri-He,Yao, Quan-Hong,Wang, Rong-Tan.

[15]Fabrication of the Monolithic Fiber for the Solid Phase Micro-extraction of Malachite Green. Chen, Jin,Yang, Jing-Hua,Zhang, Yu-Ping,Chen, Jin,Yang, Jing-Hua,Zhou, Xiao-Mao,Bai, Lian-Yang,Bai, Lian-Yang. 2015

[16]Sensitive and selective nanoplasmonic sensor by functionalized nanoporous gold nanoparticle array chip. Zhao, Fusheng,Qiu, Suyan,Li, Jingting,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan,Shih, Wei-Chuan,Qiu, Suyan. 2017

[17]Determination of residues of malachite green and leucomalachite green in Chinese soft-shelled turtle (Pelodiscus sinensis) muscle by ultra-high performance liquid chromatography-mass spectrometry. Zeng, Shaodong,Yang, Chunliang,Wang, Mingyue,Cha, Yubing,Lin, Ling. 2013

[18]Degradation of dyes using crude extract and a thermostable and pH-stable laccase isolated from Pleurotus nebrodensis. Yuan, Xianghe,Wang, Hexiang,Yuan, Xianghe,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2016

[19]Biological decolorization of malachite green by Deinococcus radiodurans R1. Lv, Guo-Ying,Cheng, Jian-Hui,Zhang, Zuo-Fa,Fan, Lei-Fa,Cheng, Jian-Hui,Chen, Xiao-Yang.

作者其他论文 更多>>

微信小程序