A multiplex RT-PCR assay for simultaneous detection of four viruses from sweet cherry
文献类型: 外文期刊
第一作者: Zong, Xiaojuan
作者: Zong, Xiaojuan;Wang, Wenwen;Wei, Hairong;Wang, Jiawei;Chen, Xin;Xu, Li;Zhu, Dongzi;Tan, Yue;Liu, Qingzhong
作者机构:
关键词: Multiplex RT-PCR;Magnetic nanoparticles;Cherry viruses;Detection
期刊名称:SCIENTIA HORTICULTURAE ( 影响因子:3.463; 五年影响因子:3.672 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection of four viruses: Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Little cherry virus-2 (LChV-2) and Cherry green ring mottle virus (CGRMV). Random hexamer primer was used for cDNA synthesis. Detection primers were designed against the genomic sequences of four viruses. The reaction conditions were firstly optimized by selecting primers combinations and standardizing the individual primer proportion and PCR protocols. Then, sensitivity of multiplex RT-PCR was evaluated. The assay could detect all four viruses in diluted cDNA (10(-6), about 8.64 x 10(-4) ng mu L-1) and RNA (10(-1), about 0.2 mu g). The reliability of the assay was evaluated by cloning and sequence alignments. The developed multiplex RT-PCR method was then used to test virus infections from field samples of sweet cherry in this paper. It will be quite helpful for plant quarantine and certification programs. To our knowledge, it is the first report of the multiplex RT-PCR assay for simultaneous detection of PNRSV, PDV, LChV-2 and CGRMV. (C) 2014 Elsevier B.V. All rights reserved.
分类号: S6
- 相关文献
作者其他论文 更多>>
-
Comprehensive metabolic profiling of dioxin-like compounds exposure in laying hens: Implications for toxicity assessment
作者:Hu, Xiaoxu;Xu, Tong;Chen, Yangsheng;Zheng, Liping;Wang, Chu;Zhang, Qinghua;Xie, Heidi Qunhui;Xu, Li;Zhao, Bin;Hu, Xiaoxu;Xu, Tong;Chen, Yangsheng;Zheng, Liping;Wang, Chu;Zhang, Qinghua;Xie, Heidi Qunhui;Xu, Li;Zhao, Bin;Zhang, Qian;Tang, Lijuan;Wang, Pu;Dong, Shujun;Wang, Ruiguo;Zhang, Su
关键词:Polychlorinated dibenzo-p-dioxins; and furans; Dioxin-like polychlorinated; biphenyls; Laying hens; Metabolomic
-
Unraveling the key structural characteristics enhancing digestion resistance of wheat starch-mung bean hull polyphenols complexes
作者:Wang, Ziyin;Xu, Lujing;Teng, Cong;Chai, Zhi;Feng, Jin;Lu, Yifei;Hu, Xindi;Ma, Kaiyang;Li, Ying;Wang, Ziyin;Li, Ying;Yuan, Xingxing;Chen, Xin
关键词:Wheat starch; Mung bean hull polyphenols; Molecular interaction; Multi-scale structure; Digestibility
-
Seaweed residue hydrolysate enhances the intestinal health, immunity and disease resistance in northern snakehead (Channa argus)
作者:Wang, Minmin;Wang, Wenwen;Tan, Suxu;Chang, Mengyang;Shi, Kunpeng;Nie, Yanzhao;Sha, Zhenxia;Guo, Lei;Xue, Hongtao;Yang, Yingming;Wang, Ya;Cao, Xiao;Sha, Zhenxia
关键词:Seaweed residue hydrolysate; Northern snakehead; Intestinal microbiota; Oxidative stress; Immune enhancer; Bacterial infection
-
Genome-wide association study and transcriptome analysis provide the SNPs and molecular insights into the visceral nodular disease tolerance in half-smooth tongue sole (Cynoglossus semilaevis)
作者:Chang, Mengyang;Shi, Kunpeng;Xia, Jinqi;Zhang, Ruixue;Li, Yishuai;Zang, Shaoqing;Tan, Suxu;Wang, Wenwen;Sha, Zhenxia;Chen, Songlin;Sha, Zhenxia;Chen, Songlin
关键词:Cynoglossus semilaevis; Visceral nodular disease; GWAS; RNA-seq; Disease tolerance; Multi omics
-
Autophagy promotes p72 degradation and capsid disassembly during the early phase of African swine fever virus infection
作者:Song, Jie;Li, Jiangnan;Li, Shuai;Zhao, Gaihong;Li, Tingting;Chen, Xin;Liu, Jia;Lai, Xinyu;Liu, Sitong;Zhou, Qiongqiong;Huang, Li;Weng, Changjiang;Li, Jiangnan;Li, Tingting;Huang, Li;Weng, Changjiang;Hu, Boli
关键词:African swine fever virus; p72; selective autophagy; Stub1; HSPA8; capsid disassembly
-
Shaker K+ channel NKT3A enhances potassium uptake and transport in tobacco (Nicotiana tabacum L.) seedlings under low potassium stress
作者:Xiang, Haiying;Xu, Li;Zhang, Jianduo;Mi, Qili;Gao, Qian;Yang, Wenwu;Huang, Haitao;Wang, Kunmiao;Zeng, Wanli;Yuan, Guang;Shi, Chuhan;Ning, Yang;Wang, Qian;Yuan, Guang;Shi, Chuhan
关键词:Tobacco; Potassium; Shaker K+ channel NKT3A; Uptake and transport
-
Multiple insights into differential Cd detoxification mechanisms in new germplasms of mung bean ( Vigna radiata L.) and potential mitigation strategy
作者:Wang, Yu;Li, Xin;Huang, Xueying;Lu, Qian;Qian, Meng;Shen, Zhenguo;Xia, Yan;Zhuang, Kai;Liu, Yanli;Peng, Yizhe;Chen, Xin;Peng, Kejian
关键词:Mung bean; Cadmium contamination; Cd 2+net influx; VrNramp5; Hairy root transformation