A multiplex RT-PCR assay for simultaneous detection of four viruses from sweet cherry

文献类型: 外文期刊

第一作者: Zong, Xiaojuan

作者: Zong, Xiaojuan;Wang, Wenwen;Wei, Hairong;Wang, Jiawei;Chen, Xin;Xu, Li;Zhu, Dongzi;Tan, Yue;Liu, Qingzhong

作者机构:

关键词: Multiplex RT-PCR;Magnetic nanoparticles;Cherry viruses;Detection

期刊名称:SCIENTIA HORTICULTURAE ( 影响因子:3.463; 五年影响因子:3.672 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed for simultaneous detection of four viruses: Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Little cherry virus-2 (LChV-2) and Cherry green ring mottle virus (CGRMV). Random hexamer primer was used for cDNA synthesis. Detection primers were designed against the genomic sequences of four viruses. The reaction conditions were firstly optimized by selecting primers combinations and standardizing the individual primer proportion and PCR protocols. Then, sensitivity of multiplex RT-PCR was evaluated. The assay could detect all four viruses in diluted cDNA (10(-6), about 8.64 x 10(-4) ng mu L-1) and RNA (10(-1), about 0.2 mu g). The reliability of the assay was evaluated by cloning and sequence alignments. The developed multiplex RT-PCR method was then used to test virus infections from field samples of sweet cherry in this paper. It will be quite helpful for plant quarantine and certification programs. To our knowledge, it is the first report of the multiplex RT-PCR assay for simultaneous detection of PNRSV, PDV, LChV-2 and CGRMV. (C) 2014 Elsevier B.V. All rights reserved.

分类号: S6

  • 相关文献

[1]Development and application of a universal and simplified multiplex RT-PCR assay to detect five potato viruses. Wen, Jingzhi,Zhang, Wei,Fan, Guoquan,Gao, Yanling,Bai, Yanju,Qiu, Cailing,Zhang, Shu,Shen, Yu,Zhang, Zhixiang,Meng, Xianxin.

[2]Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses. Tuo, Decai,Shen, Wentao,Yang, Yong,Yan, Pu,Li, Xiaoying,Zhou, Peng,Tuo, Decai. 2014

[3]Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses. Fu, Guanghua,Liu, Mengda,Zeng, Wenshu,Pu, Juan,Bi, Yuhai,Liu, Jinhua,Fu, Guanghua,Ma, Guangpeng,Liu, Jinhua. 2010

[4]Multiplex RT-PCR detection and distribution of four apple viruses in China. Ji, Z.,Zhao, X.,Duan, H.,Hui, T.,Wang, S.,Wang, Y.,Cao, K.,Ji, Z.. 2013

[5]Rapid discrimination of H5 and H9 subtypes of avian influenza viruses and Newcastle disease virus by multiplex RT-PCR. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Zhang, Ji-le,Cai, Xue-peng,Liu, Xiang-tao,Ding, Yao-zhong,Ma, Li-na,Yang, Sheng-hai,Jin, Lei,Liu, Yongsheng. 2008

[6]Simultaneous detection of wheat dwarf virus, northern cereal mosaic virus, barley yellow striate mosaic virus and rice black-streaked dwarf virus in wheat by multiplex RT-PCR. Liu, Yan,Liu, Wenwen,Wang, Xifeng,Zhang, Peipei,Massart, Sebastien.

[7]A two-step multiplex RT-PCR method for simultaneous detection of soil-borne wheat mosaic virus and wheat spindle streak mosaic virus from France. Gitton, F,Diao, A,Ducrot, O,Antoniw, JF,Adams, MJ,Maraite, H. 1999

[8]A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach. Zhao, Z.,Jiang, D.,Li, S.,Yu, Y.,Jiang, D.,Wu, Z.,Zhao, Z.. 2013

[9]A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens. Yang, Keli,Tian, Yongxiang,Zhou, Danna,Duan, Zhengying,Guo, Rui,Liu, Zewen,Yuan, Fangyan,Liu, Wei. 2017

[10]Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS. Liu, Liangliang,Leng, Juan,Yang, Xiai,Liao, Liping,Xiao, Aiping,Ma, Lei,Cen, Yin.

[11]Preparation of Tetraethylenepentamine Modified Magnetic Graphene Oxide for Adsorption of Dyes from Aqueous Solution. Tang, Xiaosheng,Tang, Xiaosheng,Tang, Xiaosheng,Tang, Ping,Liu, Liangliang,Tang, Ping. 2018

[12]Magnetically triggered drug release from nanoparticles and its applications in anti-tumor treatment. Hua, Xin,Yang, Qin,Zhang, Wanjiang,Wang, Qiudong,Dong, Zhimin,Zhang, Jiashuo,Tan, Shengnan,Smyth, Hugh D. C.. 2017

[13]Enhanced Performance of Magnetic Graphene Oxide-Immobilized Laccase and Its Application for the Decolorization of Dyes. Chen, Jing,Leng, Juan,Yang, Xiai,Liao, Liping,Liu, Liangliang,Xiao, Aiping.

[14]Magnetic molecularly imprinted polymers for the determination of beta-agonist residues in milk by ultra high performance liquid chromatography with tandem mass spectrometry. Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Liu, Hongcheng,Lin, Xin,Lin, Tao,Luo, Yinglan,Li, Qiwan,Zhang, Yulong.

[15]A reusable and sensitive biosensor for total mercury in canned fish based on fluorescence polarization. Shen, Tongfei,Yue, Qiaoli,Jiang, Xiuxiu,Wang, Lei,Xu, Shuling,Li, Haibo,Liu, Jifeng,Gu, Xiaohong,Zhang, Shuqiu.

[16]A novel immunosensor for squamous cell carcinoma antigen determination based on CdTe@Carbon dots nanocomposite electrochemiluminescence resonance energy transfer. Li, Shuhuai,Luo, Jinhui,Yang, Xinfeng,Wan, Yao,Liu, Chunhua.

[17]Optimization of ultrasound-assisted magnetic retrieval-linked ionic liquid dispersive liquid-liquid microextraction for the determination of cadmium and lead in water samples by graphite furnace atomic absorption spectrometry. Wang, Xie,Liu, Haitao,Lin, Chaowen,Pang, Liangyu,Yang, Junwei,Zeng, Qingbin.

[18]Degradation of nonylphenol polyethoxylates by functionalized Fe3O4 nanoparticle-immobilized Sphingomonas sp Y2. Bai, Naling,Wang, Sheng,Abuduaini, Rexiding,Zhao, Yuhua,Bai, Naling,Sun, Pengfei,Zhu, Xufen. 2018

[19]Simultaneous Extraction, Enrichment and Removal of Dyes from Aqueous Solutions Using a Magnetic Aqueous Micellar Two-Phase System. Wu, Shuanggen,Sun, Danyu,Li, Fenfang,Wang, Chaoyun,Yang, Yuanru,Tan, Zhijian,Wang, Chaoyun,Yang, Yuanru,Tan, Zhijian. 2017

[20]Chemiluminescent Detect of E. coli O157:H7 Using Immunological Method Based on Magnetic Nanoparticles. Li, Zhiyang,He, Nongyue,Li, Song,Liu, Hongna,Li, Xiaolong,Li, Zhiyang,He, Lei,Shi, Zhiyang,Wang, Hua,Dai, Yabin,Wang, Zhifei.

作者其他论文 更多>>