Diversity and space-time dynamics of endophytic archaea from sugar beet in the north slope of Tianshan Mountain revealed by 454 pyrosequencing and T-RFLP

文献类型: 外文期刊

第一作者: Shi, YingWu

作者: Shi, YingWu;TaPa, MuSi;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Sun, Jian;Zeng, Jun;Lin, Qing;Cao, ZhenHua;OuTi, KuEr;Li, YuGuo;Lou, Kai;Shi, YingWu;TaPa, MuSi;Li, Chun;Yang, HongMei;Zhang, Tao;Gao, Yan;Sun, Jian;Zeng, Jun;Lin, Qing;Cao, ZhenHua;OuTi, KuEr;Li, YuGuo;Lou, Kai

作者机构:

关键词: Sugar beet;Endophytic archaea;Diversity;Growth period;Biogeography;Pyrosequencing;T-RFLP

期刊名称:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.312; 五年影响因子:3.58 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Plants harbor complex and variable microbial communities. Using molecular-based techniques targeting the 16S rRNA gene, we studied the developmental stages and geographical location diversity of endophytic archaea in two locations (Shihezi and Changji) and four periods (the seedling growth, rosette formation, tuber growth and sucrose accumulation sampling periods) in the north slope of Tianshan Mountain, China. Community structure of mixed sample from 60 sugar beet plants was examined using PCR-based 454 pyrosequencing and terminal restriction fragment length polymorphism (T-RFLP). In total, 5290 archaea 16S rRNA sequences were obtained from all sugar beet samples. The most abundant archaea groups in all sugar beet were Methanococci, the miscellaneous Crenarchaeotic Group and Thermoplasmata. There was a marked difference in diversity of endophytic archaea in sugar beet for different growth periods. The greatest number of Operational T-RFLP Units (OTUs) was detected during sucrose accumulation (298) and rosette formation (282). Endophytic archaea diversity was reduced during seedling growth (128 OTUs) and tuber growth (55 OTUs). Nine OTUs were common to all four periods of growth. There were more OTUs in Shihezi than in Changji. Clustering analysis and principal component analysis of T-RFLP data revealed distinct shifts in endophytic archaea community profiles that corresponded to plant growth stage rather than geographical location. The dynamics of endophytic archaea communities were influenced by plant growth stage. To our knowledge, this is the first report that archaea has been identified as endophytes associated with sugar beet by the culture-independent approach. The results suggest that the diversity of endophytic archaea is abundant in sugar beet.

分类号: Q93

  • 相关文献

[1]Endophytic fungal diversity and space-time dynamics in sugar beet. Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai,Shi, YingWu,Li, Chun,Yang, HongMei,Zhang, Tao,Gao, Yan,Zeng, Jun,Lin, Qing,Mahemuti, Outikuer,Li, Yuguo,Huo, Xiangdong,Lou, Kai.

[2]Illumina-based analysis of endophytic bacterial diversity and space-time dynamics in sugar beet on the north slope of Tianshan mountain. Shi, YingWu,Yang, Hongmei,Zhang, Tao,Sun, Jian,Lou, Kai.

[3]Microbial diversity associated with Tricholoma matsutake fruiting bodies. Xiong, Ch,Zheng, L.,Huang, W.,Chen, Ch,Penttinen, P.,Li, Q..

[4]On-farm assessment of effect of low temperature at seedling stage on early-season rice quality. Huang, Min,Jiang, Ligeng,Zou, Yingbin,Zou, Yingbin,Zhang, Weixing. 2013

[5]Reduced dependence of rhizosphere microbiome on plant-derived carbon in 32-year long-term inorganic and organic fertilized soils. Ai, Chao,Liang, Guoqing,Sun, Jingwen,Wang, Xiubin,He, Ping,Zhou, Wei,He, Xinhua,He, Ping,He, Xinhua,He, Xinhua.

[6]Impact of brassicaceous seed meals on the composition of the soil fungal community and the incidence of Fusarium wilt on chili pepper. Ma, Yan,Gentry, Terry,Hu, Ping,Pierson, Elizabeth,Gu, Mengmeng,Yin, Shixue.

[7]Effect of fumigation with 1,3-dichloropropene on soil bacterial communities. Liu, Xiumei,Cheng, Xingkai,Wang, Kaiyun,Qiao, Kang,Wang, Hongyan.

[8]Nitrification of archaeal ammonia oxidizers in acid soils is supported by hydrolysis of urea. Lu, Lu,Wu, Yucheng,Wang, Baozhan,Lin, Xiangui,Zhu, Jianguo,Jia, Zhongjun,Lu, Lu,Han, Wenyan,Zhang, Jinbo,Cai, Zucong.

[9]Transcriptome analysis of the grass carp (Ctenopharyngodon idella) using 454 pyrosequencing methodology for gene and marker discovery. Bai, J. J.. 2015

[10]Duration-Related Variations in Archaeal Communities after a Change from Upland Fields to Paddy Fields. Jiang, Nan,Wei, Kai,Chen, Lijun,Chen, Rui.

[11]Do the intestinal microbiotas differ between paddlefish (Polyodon spathala) and bighead carp (Aristichthys nobilis) reared in the same pond?. Li, X. M.,Zhu, Y. J.,Yang, D. G.,Yan, Q. Y.,Ringo, E..

[12]Different Continuous Cropping Spans Significantly Affect Microbial Community Membership and Structure in a Vanilla-Grown Soil as Revealed by Deep Pyrosequencing. Xiong, Wu,Zhao, Jun,Xun, Weibing,Li, Rong,Zhang, Ruifu,Shen, Qirong,Xiong, Wu,Zhao, Qingyun,Wu, Huasong.

[13]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[14]Tuber turmericum sp nov., a Chinese truffle species based on morphological and molecular data. Fan, Li,Liu, Xiaohong,Cao, Jinzhong. 2015

[15]Early-diverging bumblebees from across the roof of the world: the high-mountain subgenus Mendacibombus revised from species' gene coalescents and morphology (Hymenoptera, Apidae). Williams, Paul H.,Huang, Jiaxing,An, Jiandong,Williams, Paul H.,Rasmont, Pierre.

[16]The epidemicity of facultative microsymbionts in faba bean rhizosphere soils. Xiong, Hui Yang,Zhang, Xing Xing,Guo, Hui Juan,Ji, Yuan Yuan,Li, Ying,Wang, Xiao Lin,Zhao, Wei,Mo, Fei Yu,Chen, Jin Cheng,Chen, Wen Xin,Tian, Chang Fu,Yang, Tao,Zong, Xuxiao.

[17]The bumblebees of North China (Apidae, Bombus Latreille). An, Jiandong,Huang, Jiaxing,Wu, Jie,Williams, Paul H.,Shao, Youquan,Zhang, Shiwen,Wang, Biao,Liu, Xinyu,Williams, Paul H..

[18]Low genetic differentiation among widely separated populations of the pearl oyster Pinctada fucata as revealed by AFLP. Yu, DH,Chu, KH.

[19]A molecular phylogeny of Staphyleaceae: Implications for generic delimitation and classical biogeographic disjunctions in the family. Harris, A. J.,Wen, Jun,Chen, Ping-Ting,Xu, Xin-Wei,Zhang, Jian-Qiang,Yang, Xue. 2017

[20]The biogeography of fungal communities in wetland sediments along the Changjiang River and other sites in China. Tian, Jianqing,Xiang, Meichun,Sun, Jingzu,Liu, Xingzhong,Wu, Bing,Bai, Chunming.

作者其他论文 更多>>