Immunoproteomic analysis of Schistosoma japonicum schistosomulum proteins recognized by immunoglobulin G in the sera of susceptible and non-susceptible hosts

文献类型： 外文期刊

第一作者： Hong, Yang

作者： Hong, Yang;Zhang, Min;Yang, Jianmei;Cao, Xiaodan;Han, Qian;Han, Yanhui;Qiu, Chunhui;Zhu, Chuangang;Lu, Ke;Li, Hao;Fu, Zhiqiang;Lin, Jiaojiao;Zhang, Min;Han, Yanhui;Qiu, Chunhui;Lin, Jiaojiao

作者机构：

关键词： Immunoproteomics;Microtus fortis;Schistosoma japonicum;Schistosomula;DnaJ (Hsp40) homologue

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to search for immunogenic schistosomula proteins in the hope of identifying novel intervention targets. Schistosomula proteins were analyzed by immunoproteomic which the probes were sera derived from BALB/c mice (susceptible hosts) and Microtus fortis (resistant hosts). A total of 116 immunoreactive proteins recognized by 10 days post-infected BALB/c mice, M. fortis sera, and uninfected M. fortis sera were selected for further analysis. Finally, 95 protein spots were identified by mass spectrometry (MS) analysis. Bioinformatics analysis showed that the differentially identified immunogenic proteins participated mainly in cytoskeleton organization, cell motility, energy metabolism, responses to stimuli, and protein folding. Many of these proteins were the tegument or excretory secretory products of schistosomes reported in previous studies. Among of them, Schistosoma japonicum DnaJ (Hsp40) homologue (SjDnaJ) was successfully expressed and the purified recombinant product was evaluated by immunoprotective experiment. After immunization of BALB/c mice with recombinant SjDnaJ, it could induce 34.5% and 48.9% reductions in the numbers of worms and eggs in the liver. These results contribute to a better understanding of the molecular mechanisms underlying the host parasite relationship and provide a major dataset to facilitate the further development of new vaccine candidates and/or diagnostic markers for schistosomiasis.

分类号： Q51

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