Polyploidy Induction of Mutation by Using Colchicine on Tube Seedlings of Victoria Grape

文献类型： 外文期刊

第一作者： Chang, Y. Y.

作者： Chang, Y. Y.;Ji, X.;Zhu, J. L.;Hao, Y.

作者机构：

关键词： chromosome;doubling rate

期刊名称：X INTERNATIONAL CONFERENCE ON GRAPEVINE BREEDING AND GENETICS

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收录情况： SCI

摘要： Stem segments of the tissue culture-grown seedlings of the Victoria grape were soaked 24 or 48 h using an aqueous solution of colchicine (0.05% or 0.1%). The best treatment for chromosome duplication efficiency was the use of the third and fourth budstreated for 48 h in 0.05% colchicine or 24 h in 0.1% colchicine. The doubling rates of primary generation cells were 33% and 31%; respectively. The polyploid cell percentage increases about 10% by subculture of the chimeras. The polyploid cell percentage could reach 75.8%-96.6% by subculturing the chimeras for four to six generations. Through root tip cell chromosome observations, there was little effect on the division cycle of root-tip cells in terms of the cultured seedlings with or without illumination in the short term, and the optimal sampling time of root tips for chromosome observation was at 9:30 am.

分类号： S6

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