Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China
文献类型: 外文期刊
第一作者: Cheng, Yuening
作者: Cheng, Yuening;Wang, Jianke;Zhang, Miao;Zhao, Jianjun;Shao, Xiqun;Zhao, Hang;Lin, Peng;Wu, Hua;Ma, Zengjun
作者机构:
关键词: Canine distemper virus;Raccoon dog;Genome sequence;H gene
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Canine distemper virus (CDV) is a major pathogen not only in raccoon dogs but also in a variety of carnivorous animals, including domesticated animals, particularly if they have not been vaccinated. In this study, a wild-type strain of CDV was isolated from lung tissue from a raccoon dog kept at a fur farm in Jilin Province, China. Cytopathic effects typical of CDV infection were observed after three blind passages in Vero cells, yielding a virus titer of 10(4.6) TCID50/mL. Virus identification was carried out by RT-PCR, immunofluorescence, electron microscopy, and genome sequencing. The results showed that the isolated virus, termed the SY strain, corresponded to the Asia-1 genotype of CDV and has a genome of 15,690 nucleotides. This represents the first complete nucleotide sequence of a CDV strain circulating in raccoon dogs in China.
分类号: R37
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