Identification of a highly successful cryopreservation method (droplet-vitrification) for petunia

文献类型： 外文期刊

第一作者： Zhang, Jin-Mei

作者： Zhang, Jin-Mei;Chen, Xiao-Ling;Huang, Bin;Zhou, Yuan-Chang;Zhang, Xiao-Ning;Volk, Gayle M.

作者机构：

关键词： Droplet-vitrification;Genetic stability;One-factor experiment;Orthogonal array;Petunia

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Petunia (Petunia x hybrida Vilm.) is an important horticultural crop conserved in the National Genebank of China. Here, a droplet-vitrification cryopreservation protocol was developed for petunia shoot tips. An orthogonal array experiment and additional one-factor experiments were performed to optimize key variables, including the age of in vitro plants, the concentration of sucrose in the preculture solution, the preculture duration, the duration of osmoprotection (2.0 M glycerol and 0.4 M sucrose), the length of exposure to and concentration of plant vitrification solution 2 (30% glycerol, 15% dimethyl sulfoxide, 15% ethylene glycol, and 0.4 M sucrose), and the recovery medium. By using the combined results of the orthogonal and one-factor experiments, the droplet-vitrification procedure for petunia cultivar Niu 2 was formulated efficiently and effectively. The highest regrowth levels were obtained using the following procedure: shoot tips were dissected from in vitro plantlets that were 20 d old, precultured in MS liquid medium with 0.2 M sucrose solution for 2 d, incubated with osmoprotection solution for 30 min at 25A degrees C, cryoprotected with PVS2 for 30 min at 0A degrees C, and rapidly immersed in liquid nitrogen. Cryopreserved shoot tips were then diluted in MS liquid medium with 1.2 M sucrose for 20 min at 25A degrees C and regrown on solidified MS basal medium with half concentration of NH4NO3, KH2PO4, KNO3, and sucrose. Regrowth levels were as high as 80%. No morphological changes were observed and amplification of 15 simple sequence repeats revealed no genetic alterations after cryopreservation.

分类号： Q94

  • 相关文献

[1]Identification and functional analysis of anthocyanin biosynthesis genes in Phalaenopsis hybrids. Wang, L. M.,Zhang, J.,Dong, X. Y.,Fu, Z. Z.,Jiang, H.,Zhang, H. C.. 2018

[2]The impact of elevated ozone on the ornamental features of two flowering plants (Tagetes erecta linn. Petunia hybrida Vilm.). Yang, Ning,Wang, Xiaoke,Chen, Yuanyuan,Yang, Ning,Zheng, Feixiang.

[3]Heavy metal accumulation characteristics and adaptability of different petunia varieties planted on urban green space. Zhang, Wen-Jun,Li, Xiao-Kun,Wu, Ji,Lu, Jian-Wei,Wu, Ji,Chen, Fang.

[4]Cryopreservation of Jerusalem artichoke cultivars using an improved droplet-vitrification method. Zhang, Jin-Mei,Han, Li,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Han, Li,Wang, Ling,Volk, Gayle M..

[5]Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation. Zhang, Jin-Mei,Zhang, Xiao-Ning,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Zhang, Xiao-Ning,Xu, You-Ming,Zhang, Xiao-Ning.

[6]CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD. Zhang, Jin-mei,Lu, Xin-xiong,Xin, Xia,Yin, Guang-kun,He, Juan-juan,Chen, Xiao-ling,Huang, Bin,Volk, Gayle M.. 2015

[7]DEVELOPMENT OF THREE-VITRIFICATION-BASED CRYOPRESERVATION PROCEDURES FOR SHOOT TIPS OF CHINA'S POTATO. Wang, Biao,Wang, Ren-rui,Li, Jing-wei,Ma, Yan-li,Wang, Qiao-chun,Wang, Ren-rui,Li, Min-fu,Sheng, Wan-min. 2013

[8]Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. Zhai, ZY,Wu, YJ,Engelmann, F,Chen, RZ,Zhao, YH. 2003

[9]Selection of cold tolerant somaclonal variant from Citrus sinensis cv. Jincheng and genetic stability evaluation of its cold tolerance. Lin, DB,Yan, QS,Shen, DX. 1999

[10]Mapping of quantitative trait loci for kernel row number in maize across seven environments. Lu, Ming,Xie, Chuan-Xiao,Li, Xin-Hai,Hao, Zhuan-Fang,Li, Ming-Shun,Weng, Jian-Feng,Zhang, De-Gui,Bai, Li,Zhang, Shi-Huang,Lu, Ming.

[11]Effects of Different Conservation Methods on the Genetic Stability of Potato Germplasm. Bai, J. M.,Chen, X. L.,Lu, X. X.,Xin, X.,Zhang, Z. E.,Zhang, J. M.,Bai, J. M.,Sui, Q. J.,Liu, X. C.,Sun, B. Sh..

[12]Molecular assessment of genetic identity and genetic stability in banana cultivars (Musa spp.) from China using ISSR markers. Lu, Ying,Zhang, Xin,Pu, Jinji,Qi, Yanxian,Xie, Yixian. 2011

[13]An approach for long-term preservation of Blumeria graminis f. sp tritici. Gong, Shuangjun,Yang, Lijun,Xiang, Libo,Shi, Wenqi,Xue, Minfeng,Zeng, Fansong,Zhang, Xuejiang,Wang, Hua,Yu, Dazhao.

作者其他论文 更多>>

微信小程序