Assessment of the IFN-beta response to four feline caliciviruses: Infection in CRFK cells

文献类型： 外文期刊

第一作者： Tian, Jin

作者： Tian, Jin;Zhang, Xiaozhan;Wu, Hongxia;Liu, Chunguo;Liu, Jiasen;Hu, Xiaoliang;Qu, Liandong

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关键词： IFN-beta response;Feline calicivirus

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

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收录情况： SCI

摘要： Feline calicivirus (FCV) is a highly contagious pathogen with a widespread distribution. Although the cat genome has been sequenced, little is known about innate immunity in cats, which limits the understanding of FCV pathogenesis. To investigate the IFN-beta response during FCV infection in CRFK cells, we first cloned and identified the feline IFN-beta promoter sequence and the positive regulatory domain (PRD) motifs, which shared a high similarity with human and porcine IFN-beta promoters. Next, we found that infections with FCV strains F9, Bolin and HRB-SS at the 100 or 1000 TCID50 doses could not activate the IFN-beta promoter at 12 and 24 h post-infection. Only strain 2280 infection at a 1000 TCID50 dose could induce the IFN-beta promoter mainly through IRF3 and partially through NF-kappa B, at 24 h post-infection. However, the IFN response occurred much later and was smaller in magnitude compared with that following Sendai virus (SeV) infection. Further, we found that induction of the IFN-beta promoter by FCV 2280 infection depended on dsRNA and not on viral proteins. Finally, we examined whether the IFN-beta response had an antiviral effect against FCV replication. The over-expression of IFN-beta before exposure to the virus reduced viral yields by a range of 2.2-3.2 log(10)TCID(50), but its over-expression at 12 h post-infection did not inhibit FCV replication. Our results indicate that some FCV strains cannot induce IFN-beta expression in vitro, which may be a potential factor for FCV survival in cats. Whether this is important in evading the host interferon response in vivo must be investigated. (C) 2015 Published by Elsevier B.V.

分类号： R1

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