Duck hepatitis A virus serotype 1 minigenome: a model for studying the viral 3'UTR effect on viral translation

文献类型： 外文期刊

第一作者： Liang, Ruiying

作者： Liang, Ruiying;Li, Chuanfeng;Meng, Chunchun;Chen, Zongyan;Zhu, Jie;Miao, Qiuhong;Ding, Chan;Liu, Guangqing;Liang, Ruiying;Jin, Hongyan

作者机构：

关键词： Duck hepatitis A virus;Minigenome system;3 ' UTR

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To date, the genetic replication and translation mechanisms as well as the pathogenesis of duck hepatitis A virus type 1 (DHAV-1) have not been adequately characterized due to the lack of a reliable and efficient cell culture system. Although the full-length infections clone system is the best platform to manipulate the virus, it is relatively difficult to assemble this system due to the lack of a suitable cell line. It has been proven that the minigenome system an efficient reverse genetics system for the study of RNA viruses. In some cases, it can be used to displace the infectious clone of RNA viruses. Here, we generated a minigenome for DHAV-1 with two luciferase reporter genes, firefly luciferase (Fluc) and Renilla luciferase (Rluc). The Rluc gene was used as a reference gene for the normalization of the Fluc gene expression in transfected cells, which provided a platform for studying the regulatory mechanisms of DHAV-1. Furthermore, to investigate the role of DHAV-3'UTR in the regulation of viral protein translation, deletions in the 3'UTR were introduced into the DHAV-1 minigenome. Luciferase activity, an indicator of virus translation, was then determined. These results showed that a minigenome system for DHAV-1 was successfully constructed for the first time and that the complete or partial deletion of the DHAV-3'UTR did not affect the expression level of the reporter gene, indicating that DHAV-1 translation may not be modulated by the viral genomic 3'UTR sequence.

分类号： R37

  • 相关文献

[1]Identification and Analysis of a Functional SNP 1196C > A in 3 ' UTR of Chicken IGFBP2 Gene. Yu Ying-Ying,Qiao Shu-Pei,Sun Ying-Ning,Song He,Zhang Xiao-Fei,Yan Xiao-Hong,Li Hui,Wang Ning,Yu Ying-Ying,Qiao Shu-Pei,Sun Ying-Ning,Song He,Zhang Xiao-Fei,Yan Xiao-Hong,Li Hui,Wang Ning,Yu Ying-Ying,Qiao Shu-Pei,Sun Ying-Ning,Song He,Zhang Xiao-Fei,Yan Xiao-Hong,Li Hui,Wang Ning,Sun Ying-Ning. 2014

[2]High yield expression of duck hepatitis A virus VP1 protein in Escherichia coli, and production and characterization of polyclonal antibody. Li, Chuanfeng,Chen, Zongyan,Meng, Chunchun,Li, Lu,Liu, Guangqing. 2013

[3]BmNPV-miR-415 up-regulates the expression of TOR2 via Bmo-miR-5738. Cao, Xueliang,Xia, Dingguo,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie,Zhao, Qiaoling,Huang, Yong,Cao, Xueliang,Huang, Yong,Xia, Dingguo,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie,Zhao, Qiaoling. 2017

[4]Rapid detection of duck hepatitis A virus genotype C using reverse transcription loop-mediated isothermal amplification. Li, Chuanfeng,Chen, Zongyan,Meng, Chunchun,Liu, Guangqing. 2014

[5]Genetic characterization of duck hepatitis a viruses isolated in China. Li, Jing,Bi, Yuhai,Chen, Can,Yang, Limin,Liu, Wenjun,Liu, Wenjun,Ding, Chan.

[6]Molecular cloning and characterization of a putative proline dehydrogenase gene in the Colorado potato beetle, Leptinotarsa decemlineata. Wan, Pin-Jun,Lu, Dong,Yang, Lu,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2014

[7]Duck hepatitis A virus (DHAV) genotype definition: Comment on the article by Cha et al.. Wen, Huiqiang,Han, Lingxia,Zhang, Xiaona,Lian, Chuanjiang,Zhao, Lili,Si, Changde,Chen, Hongyan. 2014

作者其他论文 更多>>

微信小程序