Yeast expressed foldable quadrivalent A beta 15 elicited strong immune response against A beta without A beta-specific T cell response in wild C57BL/6 mice

文献类型： 外文期刊

第一作者： Tan, Lin

作者： Tan, Lin;Zou, Juntao;Yao, Zhibin;Tan, Lin;Wang, Hao;Tan, Xin

作者机构：

关键词： Alzheimer disease (AD);P. pastoris;r4 x A beta 15 vaccine;T cellular immune response;C57BL/6 mice

期刊名称：HUMAN VACCINES & IMMUNOTHERAPEUTICS （ 影响因子：3.452； 五年影响因子：3.483 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Active and passive immunizations with A beta and A beta antibodies successfully reduced AD pathology and improved cognitive functions in an AD mouse model. However, human clinical trials of vaccination with synthetic A beta (AN1792), were halted due to brain inflammation, presumably induced by T cell-mediated immune response. In this study, we used Picha pastoris to produce a recombinant peptide vaccine, r4 x A beta 15 (recombinant 4 x A beta 15), four tandem repeats of A beta (1-15) interlinked by spacers. Wild-type mice were injected subcutaneously with CFA/IFA as adjuvant. r4 x A beta 15 vaccine elicited high titer anti-A beta antibodies which bound to A beta plaque in brain tissue from Tg2576 mouse. The antibody isotype was mainly IgG(1), indicating anti-inflammatory Th2 type. There was no splenocyte proliferation against A beta peptide, which indicates that the r4 x A beta 15 vaccine does not induce A beta-specific T cellular immune response. Thus, r4 x A beta 15 vaccine may be a safe and efficient vaccine for AD.

分类号： R392

  • 相关文献

[1]Expression and characterization of camel chymosin in Pichia pastoris. Wang, Nan,Li, GangQiang,Guo, WenFang,Liu, DeHu,Wang, Kevin Yueju.

[2]Inducible expression of human angiostatin by AOXI promoter in P. pastoris using high-density cell culture. Zhang, Ai-Lian,Qu, Zhi,Yi, Guo-Hui,Su, Dong-Xiao,Tu, Fa-Zhi,Pan, Ying-Wen,Zhang, Tian-Yuan,Luo, Jin-Xian,Zhang, Tian-Yuan,Luo, Jin-Xian,Fu, Ce-Yi.

作者其他论文 更多>>

微信小程序