Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

文献类型： 外文期刊

第一作者： Li, Wenjuan

作者： Li, Wenjuan;Gao, Haiyan;Li, Wenjuan;Gao, Haiyan;Mu, Honglei;Chen, Hangjun;Fang, Xiangjun;Zhou, Yongjun;Tao, Fei

作者机构：

关键词： Advanced glycation end products;Advanced lipoxidation end products;Aggregation;Amino acid modification;Conformational change;Lipid oxidation product

期刊名称：EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY （ 影响因子：2.679； 五年影响因子：2.627 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Accumulation of advanced lipoxidation end products (ALEs) has been implicated in many chronic and degenerative diseases. In this study, we evaluated the effects of malondialdehyde (MDA), trans-2-heptenal, and trans,trans-2,4-heptadienal on the properties of native proteins and the formation of bovine serum albumin (BSA)-ALEs. Incubation of BSA with these aldehydes resulted in modification of Lys and Arg residues, conformational changes, and formation of colored, fluorescent, lipofuscin-like pigments (exhibiting strong lipofuscin-like fluorescence for MDA-BSA, but much weaker fluorescence for the other two aldehyde-BSA molecules). Moreover, the generated BSA-aldehyde complexes exhibited ultraviolet-visible (UV-Vis) absorption, i.e., the MDA-BSA adduct exhibited increased UV-Vis absorbance at 290 and 400nm, while the other two aldehyde-BSA molecules exhibited increased UV-Vis absorbance at 280 and 300-350nm, respectively, and cross-linking, similar to the characteristics of Maillard reaction products. The formation of aggregates was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The variables measured above showed that heptadienal-BSA and heptenal-BSA samples exhibited both similarities and differences from MDA-BSA samples. These results provide insights into the characteristics and formation of ALEs in terms of their biochemical interactions and applications in food science. Practical applications: Advanced lipoxidation end products (ALEs) are known to be involved in many chronic and degenerative diseases. Here, we propose suitable protocols to characterize and compare ALEs derived from different secondary products of lipid oxidation. These results enhance our knowledge of the characteristics of ALEs and contribute to our understanding of the structure and formation of ALEs derived from different lipid oxidation products. Additionally, our results provide important insights into appropriate preventive or therapeutic measures to inhibit or reduce ALEs. Incubation of BSA with MDA, heptenal, and heptadienal resulted in modification of Lys and Arg residues, conformation changes, and formation of colored, fluorescent, lipofuscin-like pigments and generation of BSA-aldehyde complexes exhibiting ultraviolet-visible and cross-linking, similar to the characteristics of AGEs (Maillard reaction products).

分类号： TQ645`TS22

  • 相关文献

[1]Ability of resveratrol to inhibit advanced glycation end product formation and carbohydrate-hydrolyzing enzyme activity, and to conjugate methylglyoxal. Shen, Yixiao,Sheng, Zhanwu,Shen, Yixiao,Xu, Zhimin. 2017

[2]Cynarin-Rich Sunflower (Helianthus annuus) Sprouts Possess Both Antiglycative and Antioxidant Activities. Sun, Zheng,Chen, Feng,Sun, Zheng,Chen, Jingnan,Jiang, Yue,Ma, Jinyu,Wang, Mingfu,Ren, Guixing.

[3]Physicochemical Changes and Glycation Reaction in Intermediate-Moisture Protein-Sugar Foods with and without Addition of Resveratrol during Storage. Sheng, Zhanwu,Gu, Mantun,Hao, Wangjun,Shen, Yixiao,Zheng, Lili,Ai, Binling,Zheng, Xiaoyan,Sheng, Zhanwu,Zhang, Weimin,Xu, Zhimin.

[4]Nephroprotective effects of diacylglycerol on diabetic nephropathy in type 2 diabetic rats. Li, Jianpeng,Yan, Yongheng,Li, Xia,Liu, Zhenhua,Xu, Tongcheng,Du, Fangling,Li, Duo,Lin, Xinying,Liu, Zhenhua. 2018

[5]Major Phenolic Compounds, Antioxidant Capacity and Antidiabetic Potential of Rice Bean (Vigna umbellata L.) in China. Yao, Yang,Cheng, Xu-Zhen,Wang, Li-Xia,Wang, Su-Hua,Ren, Guixing. 2012

[6]Influence of altitudinal variation on the antioxidant and antidiabetic potential of azuki bean (Vigna angularis). Yao, Yang,Cheng, Xuzhen,Wang, Suhua,Wang, Lixia,Ren, Guixing. 2012

[7]Generation of chimeric minipigs by aggregating 4-to 8-cell-stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein. Ji, Huili,Long, Chuan,Feng, Chong,Shi, Ningning,Jiang, Yingdi,Zeng, Guomin,Li, Xirui,Wu, Jingjing,Pan, Dengke,Ji, Huili,Lu, Shengsheng,Long, Chuan,Lu, Lin. 2017

[8]The role of DNA binding with the Cry8Ea1 toxin of Bacillus thuringiensis. Song, Fuping,Zhang, Jie,Guo, Shuyuan,Li, Jie.

[9]Suspension stability and aggregation of multi-walled carbon nanotubes as affected by dissolved organic matters extracted from agricultural wastes. Li, Helian,Qiu, Yanhua,Wang, Xiaonuan,Liu, Wenhao,Ma, Yibing,Li, Helian,Chen, Guangcai,Xing, Baoshan,Chen, Guangcai,Ma, Yibing.

[10]Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta. Li, Shu Ying,Ding, Yang,Nie, Ying,Tang, Xuan Ming,Shi, Li Jun. 2015

[11]alpha(s)-casein inhibits the pressure-induced aggregation of beta-lactoglobulin through its molecular chaperone-like properties. He, Jin-Song,Zhu, Songming,Yu, Yong,Li, Jianping,He, Jin-Song,Azuma, Norihiro,Mu, Tai-Hua. 2011

[12]Study on external factors affecting egg yolk plasma by asymmetrical flow field-flow fractionation. Dou, Haiyang,Wang, Jing,Shen, Shigang,Dou, Haiyang,Zhang, Xiaoyue,Zhang, Wenhui,Li, Yueqiu,Ding, Liang,Zhang, Aixia,Choi, Jaeyeong,Lee, Seungho.

[13]The aggregation behavior of Harmonia axyridis in its native range in Northeast China. Michaud, J. P.,Wang, S.,Zhang, F.,Guo, X. J.,Tan, X. L..

[14]Molecular Size and Aggregation Behavior of Erwinia Gum in Aqueous Solution. Zhang, LN,Xu, XJ,Zhang, M,Chen, JH,Meng, DY,Ren, LW,Iijima, H.

[15]Host finding and recognition by Altica carduorum, a defoliator of Cirsium arvense. Wan, FH,Harris, P. 1996

作者其他论文 更多>>

微信小程序