Preparative isolation, quantification and antioxidant activity of dihydrochalcones from Sweet Tea (Lithocarpus polystachyus Rehd.)

文献类型： 外文期刊

第一作者： Sun, Yinshi

作者： Sun, Yinshi;Liu, Zhengbo;Li, Wei

作者机构：

关键词： Dihydrochalcone;Preparative separation;Antioxidant activity;Quantification;Sweet Tea

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： Dihydrochalcones are the main active components of Lithocarpus polystachyus Rehd. (Sweet Tea), they are directly related to the sweet tonic beverage and traditional herb. In this work, two runs of preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane/ethyl acetate/ethanol/water (1:4:3:4, v/v) were employed to separate three dihydrochalcones (phloridzin, trilobatin and phloretin) from Sweet Tea. About 6.4 mg of phloridzin, 48A mg of trilobatin, and 4.7 mg of phloretin with purities of 96.7%, 98.4% and 98.1% were obtained from 130 mg of the crude Sweet Tea extract. Phloridzin, trilobatin, and phloretin had effective radical scavenging activities, with IC50 values of 866.80, 20.16 and 179.4711g/mL, respectively, in a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method. The contents of phloridzin, trilobatin and phloretin in dried old leaves and tender leaves of tea were in the range of 10.1-18.0, 113.7-128.8,3.6-4.3 mg/g and 9.3-9.8, 82.9-103.1, 1.9-2.5 mg/g, respectively. The results indicated that the HPLC had good precision, accuracy and repeatability for the determination of three dihydrochalcones in samples. (C) 2015 Elsevier B.V. All rights reserved.

分类号： Q503

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