Molecular Markers to Detect the Formation of Heterokaryon and Homokaryon from Asexual Spores of the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes)

文献类型： 外文期刊

第一作者： Wang, Hong

作者： Wang, Hong;Cai, Tao;Wei, Jing;Feng, Aiping;Lin, Nan;Bao, Dapeng

作者机构：

关键词： medicinal mushrooms;Cordyceps militaris;asexual spore;mating-type gene;mycelial growth rate

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）

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收录情况： SCI

摘要： Cordyceps militaris is widely cultivated on artificial media in China; however, the cultures often are afflicted with the degeneration of nonfruiting strains. To understand the mechanism of degeneration of C. militaris, from the heterokaryotic strain into the homokaryotic strain, we examined the mating-type genes present in individual asexual spores. Further, we determined the distribution ratio of the different mating-type genes among a sample of asexual spores and the growth rate of heterokaryotic and homokaryotic strains of C. militaris. The distribution ratio of 3 groups of asexual spores from C. militaris heterokaryotic strains was determined as 1: 1: 1 by statistical analysis, whereas that of the two types of nuclei among asexual spores was 1: 1. Nearly two-thirds of the asexual spore isolates were homokaryon, which showed a growth speed similar to the heterokaryon. However, the homokaryon ( bearing mating-type MAT-HMG) grew significantly faster at times compared with the heterokaryon. Therefore, the purity of the spawn was difficult to establish. C. militaris heterokaryotic strains can transform into a homokaryotic strain following continued subculture.

分类号： Q94

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