Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken

文献类型： 外文期刊

第一作者： Tan, Lei

作者： Tan, Lei;Liao, Ying;Fan, Jin;Zhang, Yuqiang;Mao, Xiang;Sun, Yingjie;Song, Cuiping;Qiu, Xusheng;Meng, Chunchun;Ding, Chan;Ding, Chan

作者机构：

关键词： Infectious bronchitis virus;MHC I;Haplotype;Cytotoxic T lymphocyte;Epitopes;Immune protection

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Infectious bronchitis virus (IBV) is a major pathogen common in the poultry industry. Broad cytotoxic T lymphocyte (CTL) response against IBV is one of the crucial factors that help to control viral replication. Spike glycoproteins on the surface of the IBV virion harbor major T cell epitopes. In this study, based on the peptide-binding motifs of chicken MHC I molecules for the BF2*4, BF2*12, BF2*15, and BF2*19 haplotypes, potential CTL epitopes were predicted using S1 proteins from different IBV strains. Twenty-one peptides were predicted to be potential CTL epitopes; they were manually synthesized and the CTL responses to them tested in vitro. Spleen lymphocytes were collected from specific-pathogen free (SPF) chicken that had been immunized with the S1 protein expression plasmid, pV-51, and were stimulated by the synthesized peptides. IFN-gamma secretion and CD8(+) T cell proliferation in chickens were tested by ELISpot array and flow cytometry, respectively. Four epitopes (P8(SRIQTATOP), P9(SRNATGSQP), P18(GAYAVVNV), and P19(SRIQTATQP)) were identified to stimulate CD8(+) T cell proliferation and IFN-gamma secretion, indicating their efficacy as CTL epitopes in chicken. Poly-CTL-epitope DNA vaccine (pV-S1T) was constructed by inserting nucleotide sequences encoding the P8, P9, P18, and P19 CTL epitopes into the pVAX1 vector. Chickens were vaccinated with either pV-S1, pV-S1T, or pVAX1 and the protection efficacy was analyzed, revealing that ninety percent of chickens immunized with pV-SIT were protected after challenge with 10(6) ELD50 of IBV, demonstrating that these novel CTL epitopes were effective against IBV challenge. This study provides a new method to screen virus CTL epitopes in chicken and to develop poly-CTL-epitope DNA vaccines. (C) 2015 Elsevier Ltd. All rights reserved.

分类号： R392

  • 相关文献

[1]Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages. Xie, Xiaoli,Han, Meng,Zhang, Liang,Liu, Laixing,Gu, Zuye,Yang, Mei,Yang, Hongjun. 2016

[2]Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model. Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua,Wang, Xiao,Yao, Junhu,Wang, Xiao,Guan, Qingfeng,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Wang, Jianhua. 2015

[3]Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. Peng, Wei,Si, Wei,Yin, Lu,Liu, Huifang,Yu, Shenye,Liu, Siguo,Wang, Chunlai,Chang, Yuehong,Zhang, Zhuo,Hu, Shouping,Du, Yanfen. 2011

[4]Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (Paralichthys olivaceus). Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Xiao, Peng.

[5]The immune mechanism of Mycoplasma hyopneumoniae 168 vaccine strain through dendritic cells. Shen, Yumeng,Hu, Weiwei,Yang, Qian,Wei, Yanna,Feng, Zhixin. 2017

[6]Preparation and immunoprotection of subgroup B avian leukosis virus inactivated vaccine. Li, Xue,Dong, Xuan,Sun, Xiaolong,Li, Weihua,Zhao, Peng,Cui, Zhizhong,Li, Weihua,Wang, Xin. 2013

[7]Construction of Salmonella Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-free chicks. Tian Qiu-feng,Zhou Wei,Si Wei,Yi Fei,Hua Xin,Chen Li-ping,Liu Si-guo,Yu Shen-ye,Tian Qiu-feng,Zhou Wei,Yi Fei,Yue Min. 2018

[8]Recombinant adenovirus expressing type Asia1 foot-and-mouth disease virus capsid proteins induces protective immunity against homologous virus challenge in mice. Zhou, Guohui,Wang, Haiwei,Wang, Fang,Yu, Li.

[9]Escherichia coli outer membrane protein F (OmpF): an immunogenic protein induces cross-reactive antibodies against Escherichia coli and Shigella. Wang, Xiao,Teng, Da,Guan, Qingfeng,Mao, Ruoyu,Hao, Ya,Wang, Xiumin,Wang, Jianhua,Wang, Xiao,Yao, Junhu,Wang, Xiao,Teng, Da,Guan, Qingfeng,Mao, Ruoyu,Hao, Ya,Wang, Xiumin,Wang, Jianhua. 2017

[10]Analysis of murine B-cell epitopes on bluetongue virus 12 nonstructural protein 1. Wang HaiXiu,Sun EnCheng,Xu QingYuan,Yang Tao,Zhang Qin,Feng YuFei,Li JunPing,Lv Shuang,Sun Liang,Sun Jing,Wu DongLai.

[11]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[12]In silico prediction and in vitro identification of bluetongue virus 4 VP5 protein B-cell epitopes. Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P.,Wu, D. L.,Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P..

[13]Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology. Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Zhang, Guihong,Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Ji, Fangxiao,Cui, Jin,Zhang, Guihong,Liu, Rongchang,Zhang, Weidong.

[14]Identification of two novel BTV16-specific B cell epitopes using monoclonal antibodies against the VP2 protein. Wang, Wen-Shi,Sun, En-Cheng,Xu, Qing-Yuan,Yang, Tao,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai.

[15]Influence of Gamma Irradiation and Heat Treatment on the Immunogenicity of Five Epitopes of Pen a1. Mou, Hui,Gao, Mei-xu,Zhao, Jie,Zhao, Xin,Wang, Zhi-dong,Pan, Jia-rong.

[16]Review on the identification and role of Toxoplasma gondii antigenic epitopes. Wang, Yanhua,Wang, Guangxiang,Cai, Jianping,Yin, Hong.

[17]Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2. Ding, Peiyang,Li, Yafei,Zhou, Enmin,Zhang, Gaiping,Ding, Peiyang,Zhang, Teng,Li, Yafei,Teng, Man,Sun, Yaning,Liu, Xiao,Chai, Shujun,Jin, Qianyue,Zhang, Gaiping,Zhang, Teng,Liu, Xiao,Zhang, Gaiping,Jin, Qianyue,Zhang, Gaiping. 2017

[18]Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Wang, Wen-Shi,Sun, En-cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai. 2013

[19]Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia1. Zhang, Zhong-Wang,Zhang, Yong-Guang,Wang, Yong-Lu,Pan, Li,Fang, Yu-Zhen,Jiang, Shou-Tian,Lue, Jian-Liang,Zhou, Peng.

[20]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

作者其他论文 更多>>

微信小程序