Deletion of luxS further attenuates the virulence of the avian pathogenic Escherichia coli aroA mutant

文献类型： 外文期刊

第一作者： Han, Xiangan

作者： Han, Xiangan;Bai, Hao;Yang, Lijun;Xu, Da;Wang, Shaohui;Fan, Guobo;Zhang, Yuxi;Zuo, Jiakun;Tian, Mingxing;Ding, Chan;Yu, Shengqing;Bai, Hao;Tu, Jian;Qi, Kezong;Zhang, Yuxi

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关键词： Avian pathogenic Escherichia coli;aroA mutant;luxS;Pathogenicity

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

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收录情况： SCI

摘要： In this study, an aroA-deletion avian pathogenic Escherichia coli (APEC) mutant (strain DE17 Delta aroA) and aroA and /uxS double deletion APEC mutant (strain DE17 Delta luxS Delta aroA) were constructed from the APEC DE17 strain. The results showed that as compared to DE17 Delta aroA, the virulence of DE17 Delta luxS Delta aroA was further attenuated by 200- and 31.7-fold, respectively, in ducklings based on the 50% lethal dose. The adherence and invasion abilities of DE17 Delta luxS Delta aroA and DE17 Delta aroA were reduced by 36.5%/42.5% and 25.8%/29.3%, respectively, as compared to the wild-type strain DE17 (p < 0.05 and 0.01, respectively). Furthermore, in vivo studies showed that the bacterial loads of DE17 Delta luxS Delta aroA were reduced by 8400- and 11,333-fold in the spleen and blood of infected birds, respectively, while those of DE17 Delta aroA were reduced by 743- and 1000-fold, respectively, as compared to the wild-type strain DE17. Histopathological analysis showed both that the mutants were associated with reduced pathological changes in the liver, spleen, and kidney of ducklings, and changes in DE17 Delta luxS Delta aroA-infected ducklings were reduced to a greater degree than those infected with DE17 Delta aroA. Real-time polymerase chain reaction analysis further demonstrated that the mRNA levels of virulence-related genes (i.e., tsh, ompA, vat, iucD, pfs, fyuA, and fimC) were significantly decreased in DE17 Delta aroA, especially in DE17 Delta luxS Delta aroA, as compared to DE17 (p < 0.05). In addition, the deletion of aroA or the double deletion of aroA and luxS reduced bacterial motility.

分类号： R3

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