Effect of mevalonic acid on cholesterol synthesis in bovine intramuscular and subcutaneous adipocytes

文献类型: 外文期刊

第一作者: Liu, Xiaomu

作者: Liu, Xiaomu;You, Wei;Cheng, Haijian;Song, Enliang;Wan, Fachun;Han, Hong;Liu, Guifen;Liu, Xiaomu;You, Wei;Cheng, Haijian;Song, Enliang;Wan, Fachun;Han, Hong;Liu, Guifen;Zhang, Qingfeng

作者机构:

关键词: Mevalonic acid;3-Hydroxy-3-methylglutaryl-CoA reductase;Cholesterol;Bovine adipocytes;Meat quality

期刊名称:JOURNAL OF APPLIED GENETICS ( 影响因子:3.24; 五年影响因子:2.756 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Mevalonic acid (MVA) is a key material in the synthesis of cholesterol; indeed, intracellular cholesterol synthesis is also called the mevalonic acid pathway. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. This study suggests that MVA may play an important role in the differentiation of bovine adipose tissue in vivo. We investigated differential mRNA expression in bovine intramuscular preadipocytes (BIPs) and bovine subcutaneous preadipocytes (BSPs) by culturing cells from the longissimus dorsi muscle and subcutaneous fat tissues of Luxi yellow cattle. The morphology of lipid accumulation of bovine preadipocytes was detected by Oil Red O staining, and total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) levels were measured. Temporospatial expression of HMGR was investigated by real-time quantitative polymerase chain reaction (PCR). The TC, LDLC, and HDLC content did not significantly differ over time but increased slowly with increasing MVA concentration. HMGR expression increased over time and with increasing concentrations of MVA. MVA increased adipose cell proliferation in a dose-dependent and time-dependent manner. MVA stimulated HMGR expression in two cell types and its influence on adipocyte differentiation.

分类号: Q1

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