Molecular cloning, expression, purification and characterization of a novel cellulase gene (Bh-EGaseI) in the beetle Batocera horsfieldi

文献类型: 外文期刊

第一作者: Mei, Hui-Zhen

作者: Mei, Hui-Zhen;Xia, Ding-Guo;Zhao, Qiao-Ling;Zhang, Guo-Zheng;Qiu, Zhi-Yong;Qian, Ping;Mei, Hui-Zhen;Xia, Ding-Guo;Zhao, Qiao-Ling;Zhang, Guo-Zheng;Qiu, Zhi-Yong;Qian, Ping;Lu, Cheng;Xia, Ding-Guo

作者机构:

关键词: Batocera horsfieldi;Cellulase;Endoglucanases;Baculovirus;Silkworm

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

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页码:

收录情况: SCI

摘要: Wood-feeding insects depend heavily on the secretion of a combination of cellulases, mainly endoglucanases and other glucanases such as exoglucanases and xylanases, to achieve efficient digestion of the cellulose of cellulosic materials. In this paper, we report a novel cellulose Bh-EGaseI belonging to the glycoside hydrolase family 45(gh45-1) obtained from the beetle Batocera horsfieldi. The Bh-EGaseI gene spans 714 bp and consists of three exons coding 237 amino acid residues. The cDNA encoding Bh-EGaseI was expressed as 25 KDa in baculovirus-infected Bombyx mori larvae. The expression products of Bh-EGaseI from larval hemolymph showed a specific enzymatic activity of approximately 1030.87 IU per mg. The enzyme was active over a wide range of pH and temperatures; optimal activity was observed at 40 degrees C and pH 4.0. The effects of ions on Bh-EGaseI activity were also studied, and results indicated that activity decreased to different extents upon addition of ions. Investigations on Bh-EGasel facilitate their potential application in the production of bioenergy and biomaterials from cellulosic biomass in the future. (C) 2015 Elsevier B.V. All rights reserved.

分类号: R394

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