Saccharification of orange peel wastes with crude enzymes from new isolated Aspergillus japonicus PJ01

文献类型： 外文期刊

第一作者： Xia, Jin-lan

作者： Xia, Jin-lan;Nie, Zhen-yuan;Shan, Yang

作者机构：

关键词： Saccharification;Orange peel;Enzyme;Kinetic parameter

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

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收录情况： SCI

摘要： This study investigated the saccharification of orange peel wastes with crude enzymes from Aspergillus japonicus PJ01. Pretreated orange peel powder was hydrolyzed by submerged fermentation (SmF) and solidstate fermentation (SSF) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 degrees C were suitable saccharification conditions. The hydrolysis kinetics showed that the apparent Michaelis-Menten constant K-mapp and maximal reaction rate V-maxapp were 73.32 g/L and 0.118 g/(L min) for SmF enzyme, and 41.45 g/L and 0.116 g/(L min) for SSF enzyme, respectively. After 48 h of hydrolysis, the saccharification yields were 58.5 and 78.7 %, the reducing sugar concentrations were 14.9 and 20.1 mg/mL by SmF and SSF enzymes. Material balance showed that the SmF enzymatic hydrolysate was enriched galacturonic acid > arabinose > galactose > xylose, and the SSF enzymatic hydrolysate was enriched galacturonic acid > xylose > galactose > arabinose.

分类号： Q7

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