Comparative Study of Early Cold-Regulated Proteins by Two-Dimensional Difference Gel Electrophoresis Reveals a Key Role for Phospholipase D1 in Mediating Cold Acclimation Signaling Pathway in Rice

文献类型： 外文期刊

第一作者： Huo, Chenmin

作者： Huo, Chenmin;Zhang, Baowen;Wang, Hui;Liu, Meng;Gao, Yingjie;Sun, Daye;Tang, Wenqiang;Wang, Fawei;Zhang, Wenhua;Deng, Zhiping;Huo, Chenmin

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期刊名称：MOLECULAR & CELLULAR PROTEOMICS （ 影响因子：5.911； 五年影响因子：5.998 ）

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收录情况： SCI

摘要： To understand the early signaling steps that regulate cold responses in rice, two-dimensional difference gel electrophoresis (2-D DIGE)(1) was used to study early cold-regulated proteins in rice seedlings. Using mass spectrometry, 32 spots, which represent 26 unique proteins that showed an altered expression level within 5 min of cold treatment were identified. Among these proteins, Western blot analyses confirmed that the cellular phospholipase D 1 (OsPLD1) protein level was increased as early as 1 min after cold treatment. Genetic studies showed that reducing the expression of OsPLD1 makes rice plants more sensitive to chilling stress as well as cold acclimation increased freezing tolerance. Correspondingly, cold-regulated proteomic changes and the expression of the cold-responsive C repeat/dehydration-responsive element binding 1 (OsDREB1) family of transcription factors were inhibited in the pld1 mutant. We also found that the expression of OsPLD1 is directly regulated by OsDREB1A. This transcriptional regulation of OsPLD1 could provide positive feedback regulation of the cold signal transduction pathway in rice. OsPLD1 hydrolyzes phosphatidylcholine to produce the signal molecule phosphatidic acid (PA). By lipid-overlay assay, we demonstrated that the rice cold signaling proteins, MAP kinase 6 (OsMPK6) and OsSIZ1, bind directly to PA. Taken together, our results suggest that OsPLD1 plays a key role in transducing cold signaling in rice by producing PA and regulating OsDREB1s' expression by OsMPK6, OsSIZ1, and possibly other PA-binding proteins.

分类号： Q5

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