Halomonas urumqiensis sp nov., a moderately halophilic bacterium isolated from a saline-alkaline lake

文献类型： 外文期刊

第一作者： Zhang, Shanshan

作者： Zhang, Shanshan;Yan, Yanchun;Wang, Haisheng;Zhao, Baisuo;Pan, Jiao;Lu, Weidong;Wiegel, Jurgen

作者机构：

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A moderately halophilic, aerobic bacterium, strain BZ-SZ-XJ27(T), belonging to the genus Halomonas, was isolated from a saline-alkaline lake in the Xinjiang Uyghur Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequences and a multilocus sequence analysis using the 16S rRNA, gyrB and rpoD genes demonstrated that strain BZ-SZ-XJ27(T) represents a member of the genus Halomonas. On the basis of 16S rRNA gene sequence similarity, the closest relatives were Halomonas campaniensis 5AG(T), H. fontilapidosi 5CR(T), H. korlensis XK1(T) and H. sinaiensis ALO Sharm(T), with similarities of 96.2-97.2 %. DNA-DNA hybridization with H. korlensis CGMCC 1.6981(T) (the nearest phylogenetic neighbour) and H. campaniensis DSM 15293(T) (the highest 16S rRNA gene sequence similarity) showed relatedness values of 53 and 38 %, respectively, demonstrating the separateness of the three taxa. The bacterium stained Gram-negative and the cells were motile and rod-shaped. The strain formed creamy-white colonies and grew under optimal conditions of 1.42 M Na+ (range 0.22-4.32 M Na+), pH 8.0-8.5 (range pH 6.0-10.0) and 39 degrees C (range 4-43 degrees C). The dominant fatty acids were summed feature 8 (C-18 : (1)omega 7c/C-18 : 1 omega 6c; 36.6 %), C-16 : 0 (25.9 %) and summed feature 3 (C-16 : 1 omega 7c/C-16 : 1 omega 6c; 21.2 %). The dominant polar lipids were two unknown phospholipids, phosphatidylethanolamine and phosphatidylglycerol, and the main respiratory quinones were ubiquinone 9 (Q-9; 89 %) and ubiquinone 8 (Q-8; 10 %). The genomic DNA G+C content was 61.7 +/- 0.8 mol% (T-m). On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BZ-SZ-XJ27(T) is proposed to represent a novel species, Halomonas urumqiensis sp. nov., within the genus Halomonas of the family Halomonadaceae. The type strain is BZ-SZ-XJ27(T) (=JCM 30202(T) =CGMCC 1.12917(T)).

分类号： Q

  • 相关文献
作者其他论文 更多>>

微信小程序