Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses

文献类型： 外文期刊

第一作者： Wan, Chun-He

作者： Wan, Chun-He;Chen, Hong-Mei;Fu, Qiu-Ling;Shi, Shao-Hua;Fu, Guang-Hua;Cheng, Long-Fei;Chen, Cui-Teng;Huang, Yu;Hu, Kai-Hui

作者机构：

关键词： direct PCR;goose parvovirus;muscovy duck parvovirus;restriction fragment length polymorphism

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN：

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收录情况： SCI

摘要： A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR.

分类号： S85

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