Induction of systemic IFITM3 expression does not effectively control foot-and-mouth disease viral infection in transgenic pigs

文献类型: 外文期刊

第一作者: Zhang, Huawei

作者: Zhang, Huawei;Qian, Ping;Xu, Jinfang;Yang, Xi;Zhou, Rui;Chen, Huanchun;Li, Xiangmin;Zhang, Huawei;Qian, Ping;Xu, Jinfang;Yang, Xi;Zhou, Rui;Chen, Huanchun;Li, Xiangmin;Zheng, Haixue

作者机构:

关键词: Foot-and-mouth disease virus;Swine IFITM3;Transgenic pigs

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

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收录情况: SCI

摘要: Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, and can cause severe economic loss. Interferon-induced transmembrane (IFITM) proteins constitute a family of viral restriction factors that can inhibit the replication of several types of viruses. Our previous study showed that overexpression of swine IFITM3 (sIFITM3) impeded replication of the FMD virus (FMDV) in BHK-21 cells and mice. In this study, sIFITM3-transgenic (TG) pigs were produced by handmade cloning. Results showed that sIFITM3 was highly overexpressed in many organs of sIFITM3 TG pigs compared to wild type pigs. After a virulent FMDV strain (O/ES/2001) was intramuscularly inoculated, the sIFITM3-TG pigs showed slightly higher susceptibility to FMDV infection than wild-type pigs. Both groups displayed comparable degrees of clinical symptoms throughout the 14-day observation period. Therefore, the induction of systemic sIFITM3 expression does not protect pigs against FMDV infection. Based on these observations, we propose that a combination of interferons and vaccines be used to control FMDV infections and subsequent FMD outbreaks. (C) 2016 Elsevier B.V. All rights reserved.

分类号: S8

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