Direct Regulation of Extracellular Chitinase Production by the Transcription Factor LeClp in Lysobacter enzymogenes OH11

文献类型： 外文期刊

第一作者： Xu, Huiyong

作者： Xu, Huiyong;Chen, Hongfu;Liu, Hongxia;Qian, Guoliang;Liu, Fengquan;Xu, Huiyong;Chen, Hongfu;Liu, Hongxia;Qian, Guoliang;Liu, Fengquan;Shen, Yuemao;Du, Liangcheng;Chou, Shan-Ho;Chou, Shan-Ho;Liu, Fengquan

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期刊名称：PHYTOPATHOLOGY （ 影响因子：4.025； 五年影响因子：4.394 ）

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收录情况： SCI

摘要： Lysobacter enzymogenes is a gram-negative bacterial biological control agent that produces abundant extracellular enzymes capable of degrading the cell walls of fungal pathogens. In strain OH11, an isolate from China, the global regulator LeClp controls the production of extracellular chitinase by regulating the transcription of the chitinase-encoding gene chiA. Using a combination of bioinformatic, genetic, and biochemical methods, we show that LeClp regulates chiA transcription by directly binding to the chiA promoter region. Although LeClp appears to be important in this role, it is not the sole regulator of chiA transcription. Furthermore, the sequence analysis of putative LeClp binding sites indicated that the LeClp homolog could be involved in the regulation of extracellular chitinase production in diverse Lysobacter spp. by a mechanism similar to that in L. enzymogenes. Our findings present new insights into the molecular mechanism of LeClp in controlling extracellular chitinase activity, providing a fundamental road to elucidate how LeClp regulates the production of other extracellular lytic enzymes in L enzymogenes.

分类号： S432.1

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