MOLECULAR CHARACTERIZATION OF CYCLIN B AND ITS EXPRESSION PROFILE DURING OOGENESIS IN THE RED SWAMP CRAYFISH, PROCAMBARUS CLARKII (GIRARD, 1852) (DECAPODA, ASTACIDEA)
文献类型： 外文期刊
第一作者： Shui, Y.
作者： Shui, Y.;Xu, Z. H.;Zhou, X.;Shi, Y. H.;Zhou, X.
作者机构：
期刊名称：CRUSTACEANA （ 影响因子：0.529； 五年影响因子：0.65 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The maturation promoting factor (MPF), a complex of CDC2 (CDK1) and cyclin B, is a key regulator of controlling the G2/M phase transition in the meiotic maturation of the oocyte in multi cellular organisms. In this study, full-length cDNA of cyclin B (Pc-cyclin B) from the red swamp crayfish, Procambarus clarkii, was cloned using the degenerate RT-PCR and RACE methods. The cDNA of Pc-cyclin B is 2595 bp in length and encoding a protein of 402 amino acids, with a calculated molecular mass of 45.75 kDa. Six potential cytoplasmic polyadenylation elements (CPE) as well as one signal sequences (AATAAA) were found in the 3'-UTR location. Semi-quantitative RT-PCR analysis revealed that the amount of cyclin B mRNA was highest in the ovary, followed by the heart (P <0.05); and also significantly higher in the pre-vitellogenesis (pre-Vt) and primary-vitellogenesis (pVt) stages, while low in the tertiary-vitellogenesis and GVBD stages (P <0.05), suggesting that differential expression of Pc-cyclin B is closely related to oogonial proliferation (mitosis) and oocyte meiotic maturation in this species of crayfish.
分类号： Q96
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